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Allelopathic effect of leaf and seed extract of nutgrass (Cyperus tuberosus) on the germination of beans (Vigna unguiculata (L.) Walp)

机译:坚果草(香附子)的叶子和种子提取物对豆类(Vigna unguiculata(L.)Walp)发芽的化感作用

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The effect of leaf and seed extract of nutgrass (Cyperus tuberosus ) on the germination of cowpea (Vigna unguiculata (L.) Walp) was investigated at the microbiology laboratory of the Science Laboratory Technology Department, Federal Polytechnic Mubi, using six aqueous extracts: control (0.0?g?cm~(?3)), 0.5, 1.0 1.5, 2.0, and 3.0?g?cm~(?3). The experiment was laid out in a completely randomized design replicated three times. Results revealed that the aqueous leaf extract had more toxic effect on the germination of cowpea seed especially at the 3rd day to 9th day of germination. The concentration 1.0?g?cm~(?3) gave a significant (p ?≤?0.05) effect in the last 5?days of the germination experiment and is a better germination, while the higher concentration 3.0?g?cm~(?3) had recorded barely no germination at all from day 1 to day 13 of the experimental period. Bioassays also indicate that the inhibitory effect was proportional to the concentrations of the extracts that is the higher the concentration the stronger inhibitory effect, whereas the lower the concentration the lower stimulatory effect in some cases. This investigation therefore comparatively reveals that the leaves extract of C. tuberosus has more allelopathy than seed extract.
机译:在联邦理工大学Mubi科学实验室技术部的微生物实验室研究了胡of叶(Cyperus tuberosus)的叶和种子提取物对of豆(Vigna unguiculata(L.)Walp)发芽的影响,使用六种水提取物:对照(0.0?g?cm〜(?3)),0.5、1.0、1.5、2.0和3.0?g?cm〜(?3)。实验以完全随机的设计进行,重复了3次。结果表明,含水叶提取物对of豆种子的发芽具有更大的毒性作用,尤其是在发芽的第3天至第9天。在发芽实验的最后5天,浓度为1.0μg·cm·cm〜(〜3)产生显着(p≤≤0.05)的效果,并且发芽较好,而浓度为3.0μg·cm·cm〜(≤3)的发芽率更高。在实验期间的第1天至第13天,cm 3(?3)几乎没有记录到发芽。生物测定法还表明,抑制作用与提取物的浓度成正比,浓度越高,抑制作用越强,而浓度越低,在某些情况下刺激作用越低。因此,该研究比较揭示了iC的叶子提取物。结节比种子提取物具有更多的化感作用。

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