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Investigation of major phenolic antioxidants from Camellia sinensis fruits

机译:山茶果实中主要酚类抗氧化剂的研究

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The present study unveils major phenolic antioxidant compounds from Camellia sinensis fruits, followed by their investigation, purification and characterization using HPLC, ESI-MS and NMR studies. The spectrophotometric estimation results have clearly demonstrated that C. sinensis (tea) fruits contain up to 14% of total polyphenols (as gallic acid equivalent) and 7% of flavonoids (as quercetin equivalent) on dry weight basis. Differential solvent-mediated extractions have been performed for quantitative assessment of major phytoconstituents by RP-HPLC analysis. And the results have revealed that these fruits contain adequate amount of tea catechins (4%) along with caffeine (1%) and theanine (0.4%) on dry weight basis. Moreover, purification and characterization of major phytoconstituents such as epigallocatechin, epicatechin, epigallocatechin gallate and epicatechin gallate along with caffeine have been accomplished. Thus, it is clearly demonstrated that tea fruits could act as a possible and reliable source for obtaining major phenolic antioxidants.
机译:本研究揭露了来自中国茶树果实的主要酚类抗氧化剂化合物,然后通过HPLC,ESI-MS和NMR研究对其进行了研究,纯化和表征。分光光度估计结果清楚地表明了C。以干重计,中华(茶)果实含有高达14%的总多酚(以没食子酸当量计)和7%的黄酮类(以槲皮素当量计)。已经进行了差异溶剂介导的提取,以通过RP-HPLC分析定量评估主要植物成分。结果表明,这些水果含有足够量的茶儿茶素(4%)以及咖啡因(1%)和茶氨酸(0.4%)(以干重计)。而且,已经完成了主要植物成分如表没食子儿茶素,表儿茶素,表没食子儿茶素没食子酸酯和表儿茶素没食子酸酯以及咖啡因的纯化和表征。因此,清楚地证明了茶果可以作为获得主要酚类抗氧化剂的可能和可靠的来源。

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