• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cogent Chemistry >Facile synthesis of biodegradable affinity-purified lectin nanoparticles
【24h】

Facile synthesis of biodegradable affinity-purified lectin nanoparticles

机译：易于合成的可生物降解的亲和纯化的凝集素纳米粒子

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lectins are proteins/glycoproteins that exhibit distinct sugar specificities and interact with cell surface molecules and have therefore being used as potential tools for drug delivery systems. In the present study, we purified different sugar-specific lectins by affinity chromatography. A glucose/mannose-specific lectin (DLL-I) as well as galactose-specific lectin (DLL-II) [from the seeds of Dolichos lablab ], lactose-specific lectin (LSL) [from the fresh water mussel Lamellidens corrianus ] and wheat germ agglutinin (WGA) [from wheat germ seeds] were affinity purified. Purified DLL-I migrated as a pentamer (α-1, α-2, α-3, α-4 in the range of 12–20?kDa and β subunit), DLL-II migrated as a dimer (α and β, 31?kDa and 29?kDa), LSL migrated as three subunit protein in SDS-PAGE (29?kDa, 28?kDa, and 26?kDa), and WGA migrated as single subunit (18?kDa). Nanoparticles of these lectins were prepared and characterized for the size and shape by transmission electron microscopy, scanning electron microscopy, and atomic force microscopy. The DLL-I, DLL-II, LSL, and WGA nanoparticles sizes are in the range 90?±?20?nm, 150?±?20?nm, 145?±?10?nm, and 75?±?15?nm, respectively.
机译：凝集素是蛋白质/糖蛋白，具有不同的糖特异性并与细胞表面分子相互作用，因此被用作药物输送系统的潜在工具。在本研究中，我们通过亲和色谱法纯化了不同的糖特异性凝集素。葡萄糖/甘露糖特异性凝集素（DLL-I）以及半乳糖特异性凝集素（DLL-II）[来自Dolichos lablab的种子]，乳糖特异性凝集素（LSL）[来自淡水贻贝<对[Lamellidens corrianus]和小麦胚芽凝集素（WGA）[来自小麦胚芽]进行亲和纯化。纯化的DLL-I迁移为五聚体（α-1，α-2，α-3，α-4在12–20？kDa和β亚基范围内），DLL-II迁移为二聚体（α和β， 31？kDa和29？kDa），LSL在SDS-PAGE中以三个亚基蛋白（29？kDa，28？kDa和26？kDa）迁移，而WGA作为单个亚基（18？kDa）迁移。制备这些凝集素的纳米颗粒，并通过透射电子显微镜，扫描电子显微镜和原子力显微镜表征尺寸和形状。 DLL-I，DLL-II，LSL和WGA纳米粒子的尺寸范围为90？±？20？nm，150？±？20？nm，145？±？10？nm和75？±？15？nm。纳米。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号