Live Mice from Cryopreserved Embryos Derived In Vitro with Cryopreserved Ejaculated Spermatozoa

Songsasen N.; Leibo S. P.

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Live Mice from Cryopreserved Embryos Derived In Vitro with Cryopreserved Ejaculated Spermatozoa

机译：冷冻保存的精子的离体体外衍生的冷冻保存的活体小鼠

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Thisstudywasundertakentotrytoreducethenumberofanimalsrequiredtomaintainmousestrainsbybankingofembryosorspermatozoa.Theprincipalobjectivewastocryopreserveejaculatedmousespermatozoa,usingamethodrecentlydevelopedforepididymalspermatozoa.Within30minaftermating,ejaculatedspermatozoawereflushedfromtheuterusofmatedfemales;shortlyafterwards,epididymalspermatozoawerealsocollectedfromthesamemalesthathadmatedwiththefemales.Theaveragevaluesforspermatozoalmotilityandviabilityofejaculatedspecimensofninemaleswere43and46%,respectively,andforepididymalspecimens,thecorrespondingvalueswere60and52%.Inexperiment1,ejaculatedorepididymalspermatozoawereincubatedwithoocytesfor0.5to4h.Asevidencedbydevelopmentintotwo-cellembryoswithin24h,kineticsoffertilizationofthetwospermatozoatypesweresimilar.Inexperiment2,ejaculatedandepididymalspermatozoaofthreemaleswereseparatelycryopreservedinmediumcontainingraffinose,glycerol,andeggyolk.Sampleswerecooledandseededat-4°C,cooledto-70°Cat20°C/min,andthenwereplacedintoliquidnitrogenforstorage.Whencryopreservedepididymalorejaculatedspermatozoawerethawedat1,000°C/minandusedforinvitrofertilization,60%ofoocytescleaved,andapproximately95%ofcleavedembryosdevelopedintomorulaeorblastocysts.Whenembryosproducedwithcryopreservedspermatozoaweretransferredintorecipients,18and22livepupswereobtainedfrom62and54embryosresultingfromejaculatedorepididymalspermatozoa,respectively.Thisstudydocumentedthefeasibilityofcryopreservingejaculatedspermatozoaasaneffectivealternativetopreservinggermplasmfromgeneticallyvaluablemice.

机译：Thisstudywasundertakentotrytoreducethenumberofanimalsrequiredtomaintainmousestrainsbybankingofembryosorspermatozoa.Theprincipalobjectivewastocryopreserveejaculatedmousespermatozoa，usingamethodrecentlydevelopedforepididymalspermatozoa.Within30minaftermating，ejaculatedspermatozoawereflushedfromtheuterusofmatedfemales; shortlyafterwards，epididymalspermatozoawerealsocollectedfromthesamemalesthathadmatedwiththefemales.Theaveragevaluesforspermatozoalmotilityandviabilityofejaculatedspecimensofninemaleswere43and46％，分别andforepididymalspecimens，thecorrespondingvalueswere60and52％.Inexperiment1，ejaculatedorepididymalspermatozoawereincubatedwithoocytesfor0.5to4h.Asevidencedbydevelopmentintotwo-cellembryoswithin24h，kineticsoffertilizationofthetwospermatozoatypesweresimilar.Inexperiment2，ejaculatedandepididymalspermatozoaofthreemaleswereseparatelycryopreservedinmediumcontainingraffinose，甘油，andeggyolk.Sampleswerecooledandseededat-4°C，以20°C / min的速度冷却至70°C，然后将其替换为液态itrogenforstorage.Whencryopreservedepididymalorejaculatedspermatozoawerethawedat> 1,000℃/ minandusedforinvitrofertilization，> 60％ofoocytescleaved，andapproximately95％ofcleavedembryosdevelopedintomorulaeorblastocysts.Whenembryosproducedwithcryopreservedspermatozoaweretransferredintorecipients，18and22livepupswereobtainedfrom62and54embryosresultingfromejaculatedorepididymalspermatozoa，respectively.Thisstudydocumentedthefeasibilityofcryopreservingejaculatedspermatozoaasaneffectivealternativetopreservinggermplasmfromgeneticallyvaluablemice。

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《Comparative Medicine》 |1998年第3期|共页
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Songsasen N.; Leibo S. P.;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Live mice from cryopreserved embryos derived in vitro with cryopreserved ejaculated spermatozoa [J] . Songsasen N, Leibo SP Laboratory Animal Science . 1998,第3期

机译：冷冻保存的射精精子体外冷冻保存的胚胎的活小鼠
2. Live offspring from cryopreserved embryos following in vitro growth, maturation and fertilization of oocytes derived from preantral follicles in mice. [J] . Motohashi H. H., Sankai T., Kada H. The Journal of Reproduction and Development . 2011,第6期

机译：在小鼠中，从胚胎前卵泡衍生的卵母细胞在体外生长，成熟和受精后，从冷冻保存的胚胎中获得活后代。
3. Live Offspring from Cryopreserved Embryos Following In Vitro Growth, Maturation and Fertilization of Oocytes Derived from Preantral Follicles in Mice [J] . Hideyuki H. MOTOHASHI, Tadashi SANKAI, Hidemi KADA The Journal of Reproduction and Development . 2011,第6期

机译：冷冻保存的胚胎的活后代在小鼠的前壁卵泡的卵母细胞的体外生长，成熟和受精后
4. In vitro metabolism of zapotin from Casimiroa edulis in human liver microsomes and cryopreserved human hepatocytes [C] . Jinghu Li, Mark Cushman, John M. Pezzuto, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：来自人肝微粒体的Casimiroa Edulis的Zapotin的体外代谢和冷冻保存人肝细胞
5. Resazurin Reduction Assay to Evaluate the Quality of Thawed Cryopreserved Stallion Spermatozoa Motility. [D] . Genetti, Melissa Ann. 2013

机译：刃天青还原还原法评估解冻的冷冻保存的种马精子活动性的质量。
6. Live birth following transfer of a cryopreserved embryo generated from a cryopreserved oocyte and a cryopreserved sperm: Case report [O] . Debra A. Gook, Lyndon Hale, David H. Edgar 2007

机译：冷冻保存的卵母细胞和冷冻保存的精子产生的冷冻保存胚胎转移后的活产：病例报告
7. Live Offspring from Cryopreserved Embryos Following In Vitro Growth, Maturation and Fertilization of Oocytes Derived from Preantral Follicles in Mice [O] . Hideyuki H. MOTOHASHI, Tadashi SANKAI, Hidemi KADA 2011

机译：在体外生长后，冷冻保存胚胎的实时后代，卵母细胞的成熟和施肥源自小鼠的卵泡

Live Mice from Cryopreserved Embryos Derived In Vitro with Cryopreserved Ejaculated Spermatozoa

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