Cloning of the Full-Length cDNA of Porcine Antithrombin III and Comparison with its Human Homolog

Chen Younan; Tan Weidong; Qin Shengfang; Zhang Jie; Bu Hong; Li Youping; Lu Yanrong; Cheng Jingqiu

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Cloning of the Full-Length cDNA of Porcine Antithrombin III and Comparison with its Human Homolog

机译：猪抗凝血酶III全长cDNA的克隆及其与人类同源物的比较

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ThecharacterizationofporcineantithrombinIII(ATIII)#8212;ahighlypowerfulanticoagulant#8212;isessentialforusingporcineliverinxenotransplantationapplications.TheobjectiveofthisstudywastoclarifythefunctionsofporcineATIIIthroughcomparisonwithhumanATIII.WeclonedporcineATIIIandcompareditsimportantfunctionalsiteswiththoseofhumanATIII.Thefull-lengthcDNAofporcineATIIIwasclonedbyscreeningaporcinelivercDNAlibrary,andtheATIIIactivitiesof23pigsweredetermined.Thefull-lengthcDNAofporcineATIIIspanned1498bpandencoded463aminoacids.PorcineATIIIshared87.67%nucleotideidentityand89.06%aminoacididentitywithhumanATIII.CompleteidentitywasfoundatactivecenterArg393#8211;Ser394,andremarkablyhighsimilaritieswerefoundat2criticalheparin-bindingsites(residues41through49and114through156)andinsomekeyresiduesinvolvedinheparinbinding.AnATIIIassayfoundnosignificantdifferencebetweenporcineandhumanplasma.ThehighlevelofsimilaritybetweenporcineATIIIandhumanATIIIsuggeststhatporcineATIIIwillfunctioninamannersimilartohumanATIIIinxenotransplantation.

机译：ThecharacterizationofporcineantithrombinIII（ATIII）＃8212; ahighlypowerfulanticoagulant＃8212; isessentialforusingporcineliverinxenotransplantationapplications.TheobjectiveofthisstudywastoclarifythefunctionsofporcineATIIIthroughcomparisonwithhumanATIII.WeclonedporcineATIIIandcompareditsimportantfunctionalsiteswiththoseofhumanATIII.Thefull-lengthcDNAofporcineATIIIwasclonedbyscreeningaporcinelivercDNAlibrary，andtheATIIIactivitiesof23pigsweredetermined.Thefull-lengthcDNAofporcineATIIIspanned1498bpandencoded463aminoacids.PorcineATIIIshared87.67％nucleotideidentityand89.06％aminoacididentitywithhumanATIII.CompleteidentitywasfoundatactivecenterArg393＃8211; Ser394，andremarkablyhighsimilaritieswerefoundat2criticalheparin-bindingsites（residues41through49and114through156）andinsomekeyresiduesinvolvedinheparinbinding猪与人血浆之间没有相似性的ATIII分析。猪ATIII与人ATIII之间的高度相似性表明，猪ATIII的作用与人ATIII相似异种移植。

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《Comparative Medicine》 |2009年第4期|共页
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Chen Younan; Tan Weidong; Qin Shengfang; Zhang Jie; Bu Hong; Li Youping; Lu Yanrong; Cheng Jingqiu;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Cloning of the full-length cDNA of porcine antithrombin III and comparison with its human homolog [J] . Chen Y, Tan W, Qin S, Comparative Medicine . 2009,第4期

机译：猪抗凝血酶III全长cDNA的克隆及其与人同源性的比较
2. Structure of a full-length cDNA clone for the preproα2(I) chain of human type I procollagen. Comparison with the chicken gene confirms unusual patterns of gene conservation [J] . D J Prockop, G Tromp, H Kuivaniemi, The biochemical journal . 1988,第3期

机译：人I型胶原原preproα2（I）链的全长cDNA克隆的结构。与鸡基因的比较证实了基因保存的异常模式
3. Cloning of Porcine Platelet Glycoprotein Ib alpha and Comparison with the Human Homolog [J] . Shi Meimei, Qiao Jianlin, Shen Yang, Comparative Medicine . 2012,第4期

机译：猪血小板糖蛋白Ibα的克隆及其与人类同源物的比较
4. An Integrated Database System for Full-Length Human cDNA in NEDO Full-Length Human cDNA Sequencing Project [C] . Tetsuo Nishikawa, Kouichi Kimura, Tomohiro Yasuda, Workshop on Genome Informatics . 2002

机译：NEDO全长人cDNA测序项目全长人cDNA集成数据库系统
5. Generation of full-length cDNA clone and functional analysis of leader proteases of Grapevine leafroll-associated virus-2. [D] . Liu, Yu-Ping. 2009

机译：葡萄叶片相关病毒2的全长cDNA克隆的产生和前导蛋白酶的功能分析。
6. Cloning of the Full-Length cDNA of Porcine Antithrombin III and Comparison with its Human Homolog [O] . Younan Chen, Weidong Tan, Shengfang Qin, 2009

机译：猪抗凝血酶III全长cDNA的克隆及其与人类同源物的比较
7. Isolation and sequence characterization of a cDNA clone of human antithrombin III. [O] . Chandra, T, Stackhouse, R, Kidd, V J, 1983

机译：人抗凝血酶III cDNA克隆的分离和序列表征。

Cloning of the Full-Length cDNA of Porcine Antithrombin III and Comparison with its Human Homolog

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