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Cloning of the full-length cDNA of porcine antithrombin III and comparison with its human homolog

机译：猪抗凝血酶III全长cDNA的克隆及其与人同源性的比较

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摘要

The characterization of porcine antithrombin III (ATIII)-a highly powerful anticoagulant-is essential for using porcine liver in xenotransplantation applications. The objective of this study was to clarify the functions of porcine ATIII through comparison with human ATIII. We cloned porcine ATIII and compared its important functional sites with those of human ATIII. The full-length cDNA of porcine ATIII was cloned by screening a porcine liver cDNA library, and the ATIII activities of 23 pigs were determined. The full-length cDNA of porcine ATIII spanned 1498 bp and encoded 463 amino acids. Porcine ATIII shared 87.67% nucleotide identity and 89.06% amino acid identity with human ATIII. Complete identity was found at active center Arg393-Ser394, and remarkably high similarities were found at 2 critical heparin-binding sites (residues 41 through 49 and 114 through 156) and in some key residues involved in heparin binding. An ATIII assay found no significant difference between porcine and human plasma. The high level of similarity between porcine ATIII and human ATIII suggests that porcine ATIII will function in a manner similar to human ATIII in xenotransplantation.
机译：猪抗凝血酶III（ATIII）（一种高度有效的抗凝剂）的特性对于在异种移植中使用猪肝至关重要。本研究的目的是通过与人ATIII的比较来阐明猪ATIII的功能。我们克隆了猪ATIII，并将其重要的功能位点与人ATIII进行了比较。通过筛选猪肝脏cDNA文库，克隆了猪ATIII的全长cDNA，并测定了23只猪的ATIII活性。猪ATIII的全长cDNA跨度为1498bp，编码463个氨基酸。猪ATIII与人ATIII具有87.67％的核苷酸同一性和89.06％的氨基酸同一性。在活性中心Arg393-Ser394中发现了完全同一性，并且在2个关键肝素结合位点（残基41至49和114至156）和一些参与肝素结合的关键残基中发现了极高的相似性。 ATIII分析发现猪血浆和人血浆之间无显着差异。猪ATIII与人ATIII之间的高度相似性表明，猪ATIII在异种移植中将以类似于人ATIII的方式起作用。

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