Poor Quality of Oocytes from Xenopus laevis Used in Laboratory Experiments: Prevention by Use of Antiseptic Surgical Technique and Antibiotic Supplementation

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Poor Quality of Oocytes from Xenopus laevis Used in Laboratory Experiments: Prevention by Use of Antiseptic Surgical Technique and Antibiotic Supplementation

机译：用于实验的非洲爪蟾卵母细胞质量低下：通过使用抗菌外科手术技术和补充抗生素进行预防

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BackgroundandPurpose:Episodicphasesofcontinuouspoor-qualityoocytesobtainedfromSouthAmericanClawedFrogs(Xenopuslaevis)oftenareobserved.Inpublicationsdealingwiththesurgicaltechniqueofoocyteremoval,thefrogs'robustconstitutionandresistanceagainstinfectionsprovidedbymagaininsarepointedout.Forthisreason,cleanratherthansterileconditionsforthesurgicalprocedurearemostlyrecommended.However,inmostinstances,antibioticsareaddedtothebuffermediumwheninvitroexperimentsareperformedusingoocytes.brxmlns="http://pub2web.metastore.ingenta.coms/"/brMethods:Afteralongphaseofpooroocytequalityatourfacility,involvingoocytesthathadbeenobtainedbyuseofa#8220;clean#8221;surgicalprocedure,wesubsequentlyculturedoocytesinabuffermediumcontainingthethreeantibiotics:penicillinG,gentamicin,andstreptomycin.brxmlns="http://pub2web.metastore.ingenta.coms/"/brResults:DuringDNAinjectionexperiments,theoocytesdevelopedblackspotsontheirsurfacebypostoperativedaytwo.Purecultureofthegram-negativenon-fermentativerodPseudomonasfluorescenswasobtainedfromtheimpairedoocytes;theisolatewasresistanttothethreeantibiotics.Bycontrast,afterasepticsurgicalremovalandcultureofoocytesinbuffermediumcontainingtheantibioticstetracyclineandgentamicin,perfectoocyteswithoutbacterialcontaminationwereobtained.brxmlns="http://pub2web.metastore.ingenta.coms/"/brConclusion:Wheneverimpairedoocytequalityisobserved,microbialcontaminationshouldbeconsideredasapossiblecause.

机译：BackgroundandPurpose：Episodicphasesofcontinuouspoor-qualityoocytesobtainedfromSouthAmericanClawedFrogs（Xenopuslaevis）oftenareobserved.Inpublicationsdealingwiththesurgicaltechniqueofoocyteremoval，thefrogs'robustconstitutionandresistanceagainstinfectionsprovidedbymagaininsarepointedout.Forthisreason，cleanratherthansterileconditionsforthesurgicalprocedurearemostlyrecommended.However，inmostinstances，antibioticsareaddedtothebuffermediumwheninvitroexperimentsareperformedusingoocytes 方法：在工厂完成卵母细胞质量检测后，通过使用＃8220;干净的＃8221;外科手术步骤获得的涉及的卵细胞，随后在含有三种抗生素的青霉素缓冲液，庆大霉素和链霉素中缓冲培养双核细胞。术后第二天，它们的表面形成了黑点。纯培养革兰氏阴性，非发酵棒通过无菌方法在含有抗生素四环素和庆大霉素的缓冲液中对卵母细胞进行无菌外科培养后，获得具有无细菌污染的完整细胞。

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《Comparative Medicine》 |2000年第2期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Poor quality of oocytes from Xenopus laevis used in laboratory experiments: prevention by use of antiseptic surgical technique and antibiotic supplementation [J] . Holger-Andreas Elsner, Hans-Hinrich Honck, Frank Willmann, Comparative Medicine . 2000,第2期

机译：实验室实验中所用非洲爪蟾的卵母细胞质量差：通过使用无菌手术技术和补充抗生素进行预防
2. The transoocyte voltage clamp: a non-invasive technique for electrophysiological experiments with Xenopus laevis oocytes. [J] . Cucu D, Simaels J, Jans D, Pfluegers Archiv: European Journal of Physiology . 2004,第6期

机译：跨卵细胞电压钳：非洲爪蟾卵母细胞电生理实验的一种非侵入性技术。
3. Toward the understanding of biology of oocyte life cycle in Xenopus Laevis Xenopus Laevis : No oocytes left behind [J] . Reproductive medicine and biology . 2020,第2期

机译：为了了解Xenopus Laevis Xenopus Laevis的卵母细胞生命周期生物学的理解：留下了卵母细胞
4. 3D-Printed Bioresorbable Antibiotic Spacer Clip for the Prevention of Spinal Surgical Site Infection [C] . Jaclyn Schachtner, Rochitha Nathan, Manisha Rajaghatta, Antimicrobial Combination Devices Conference . 2019

机译：用于预防脊神经遗址感染的3D印刷生物可吸收抗生素夹
5. INCENP translated during oocyte maturation is a maternal factor of Xenopus laevis development. [D] . Leblond, Geoffrey. 2011

机译：卵母细胞成熟过程中翻译的INCENP是非洲爪蟾发育的母体因素。
6. Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs. [O] . M M Bendig, J G Williams 1984

机译：注射到非洲爪蟾卵母细胞和未受精卵中的非洲爪蟾球蛋白基因的转录保真度。
7. Accumulation of Annulate Lamellae in the Subcortical Layer during Progesterone-induced Oocyte Maturation in Xenopus Laevis. (annulate lamellae/oocyte maturation/Xenopus laevis) [O] . HIROSHI IMOH, MITSUMASA OKAMOTO, GORO EGUCHI 1983

机译：黄体酮诱导卵蟾卵卵卵卵卵细胞成熟过程中的增压层中的增荷。（共用薄片/卵母细胞成熟/ Xenopus Laevis）

Poor Quality of Oocytes from Xenopus laevis Used in Laboratory Experiments: Prevention by Use of Antiseptic Surgical Technique and Antibiotic Supplementation

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