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首页> 外文期刊>Comparative Medicine >Poor quality of oocytes from Xenopus laevis used in laboratory experiments: prevention by use of antiseptic surgical technique and antibiotic supplementation
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Poor quality of oocytes from Xenopus laevis used in laboratory experiments: prevention by use of antiseptic surgical technique and antibiotic supplementation

机译：实验室实验中所用非洲爪蟾的卵母细胞质量差：通过使用无菌手术技术和补充抗生素进行预防

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Background and Purpose: Episodic Phases of continuous poor-quality oocytes obtained from South American Clawed Frogs (Xenopus laevis) often are observed. In publications dealing with the surgical technique of oocyte removal, the frogs' robust constitution and resistance against infections provided by magainins are pointed out. For this reason, clean rather than sterile conditions for the surgical procedure are mostly recommended. However, in most instances, antibiotics are added to the buffer medium when in vitro experiments are performed using oocytes. Methods: After a long phase of poor oocyte quality at our facility, involving oocytes that had been obtained by use of a "clean"surgical procedure, we subsequently cultured oocytes in a buffer medium containing the three antibiotics: penicillin G, gentamicin, and streptomycin. Results: During DNA injection experiments, the oocytes developed black spots on their surface by postoperative day two. Pure culture of the gram-negative non-fermentative rod Pseudomonas fluorescens was obtained from the impaired oocytes; the isolate was resistant to the three antibiotics. By contrast, after aseptic surgical removal and culture of oocytes in buffer medium containing the antibiotics tetracycline and gentamicin, perfect oocytes without bacterial contamination were obtained. Conclusion: Whenever impaired oocyte quality is observed, microbial contamination should be considered as a possible cause.
机译：背景与目的：经常观察到从南美爪蛙（Xenopus laevis）获得的连续质量差的卵母细胞的阶段性阶段。在涉及去除卵母细胞的手术技术的出版物中，指出了青蛙的健壮构造和对抵抗由麦角菌素提供的感染的抵抗力。因此，通常建议在手术过程中使用干净而非无菌的条件。但是，在大多数情况下，使用卵母细胞进行体外实验时，会将抗生素添加到缓冲液中。方法：在我们的工厂长期经历了不良的卵母细胞质量（涉及通过“清洁”手术程序获得的卵母细胞）之后，我们随后在含有三种抗生素（青霉素G，庆大霉素和链霉素）的缓冲液中培养卵母细胞。结果：在DNA注射实验中，卵母细胞在术后第二天就在其表面形成了黑点。从受损的卵母细胞中获得革兰氏阴性非发酵棒荧光假单胞菌的纯培养物。分离株对三种抗生素具有抗性。相反，在无菌外科手术去除卵母细胞并在含有抗生素四环素和庆大霉素的缓冲液中培养卵母细胞之后，获得了无细菌污染的完美卵母细胞。结论：每当观察到卵母细胞质量受损时，应将微生物污染视为可能的原因。

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