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An Efficient Bioemulsifier-Producing Bacillus subtilis UCP 0146 Isolated from Mangrove Sediments

机译:从红树林沉积物中分离出高效的生产生物乳化剂的枯草芽孢杆菌UCP 0146

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In this work, we investigated the potential of Bacillus subtilis UCP 0146 in the bioconversion of a medium containing 100% cassava flour wastewater to obtain a bioemulsifier. The evaluation of the production was carried out by the emulsification index (IE24) and the surface tension (ST). The ionic charge, stability (temperature, salinity, and pH measured by IE24 and viscosity), and ability to remove and disperse oil and textile dye were investigated. B. subtilis produced an anionic bioemulsifier in the medium containing 100% cassava wastewater under Condition 4 of the factorial design (inoculum 9% at a temperature of 35 ?°C and shaken at 100 rpm), and showed a surface tension of 39 mN/m, an IE24 of 95.2%, and a yield of 2.69 g?·La??1. The bioemulsifier showed stability at different pH (2a??8), temperatures (0a??120 ?°C), and NaCl concentrations, a dispersion oil displacement area (ODA) test of 55.83 cm2, and a reduction of the viscosity of the burned engine oil (90.5 Cp). The bioemulsifier was able to remove petroleum (94.4%) and methylene blue azo dye (62.2%). The bioemulsifier and its synthesis from bacteria also emphasizes the role of surfactants in oil remediation.
机译:在这项工作中,我们研究了枯草芽孢杆菌UCP 0146在含100%木薯粉废水的培养基生物转化中获得生物乳化剂的潜力。通过乳化指数(IE24)和表面张力(ST)进行生产评价。研究了离子电荷,稳定性(通过IE24和粘度测量的温度,盐度和pH)以及去除和分散油和纺织品染料的能力。枯草芽孢杆菌在析因设计的条件4下(在35°C的温度下接种率为9%,在100 rpm摇动下)在含有100%木薯废水的培养基中生产了一种阴离子生物乳化剂,其表面张力为39 mN / m,IE24为95.2%,产率为2.69g·La·La 1。该生物乳化剂在不同的pH(2a ?? 8),温度(0a ?? 120°C)和NaCl浓度下显示出稳定性,分散油驱替面积(ODA)测试为55.83 cm2,并且粘度降低。燃烧机油(90.5 Cp)。该生物乳化剂能够除去石油(94.4%)和亚甲基蓝偶氮染料(62.2%)。生物乳化剂及其从细菌中的合成也强调了表面活性剂在油脂修复中的作用。

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