Assessment of Blood Collection from the Lateral Saphenous Vein for Microfilaria Counts in Mongolian Gerbils (Meriones unguiculatus) Infected with Brugia pahangi

Alworth Leanne C; Berghaus Roy D; Kelly Lisa M; Supakorndej Prasit; Burkman Erica J; Savadelis Molly D; Cooper Tanya L; Salyards Gregory W; Harvey Stephen B; Moorhead Andrew R

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Assessment of Blood Collection from the Lateral Saphenous Vein for Microfilaria Counts in Mongolian Gerbils (Meriones unguiculatus) Infected with Brugia pahangi

机译：从大隐静脉感染的蒙古沙鼠（Meriones unguiculatus）的侧隐静脉收集的微丝aria计数的评估。

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TheNIHguidelinesforsurvivalbleedingofmiceandratsnotethatusingtheretroorbitalplexushasagreaterpotentialforcomplicationsthandoothermethodsofbloodcollectionandthatthisprocedureshouldbeperformedonanesthetizedanimals.Lateralsaphenousveinpuncturehasalowpotentialforcomplicationsandcanbeperformedwithoutanesthesia.Mongoliangerbils(Merionesunguiculatus)arethepreferredrodentmodelforfilarialparasiteresearch.Tomonitormicrofilariacountsintheblood,bloodsamplingfromtheorbitalplexushasbeenthestandard.Ourgoalwastorefinethebloodcollectiontechnique.Todeterminewhetherbloodcollectionfromthelateralsaphenousveinwasafeasiblealternativetoretroorbitalsampling,wecomparedmicrofilariacountsinbloodsamplescollectedbybothmethodsfrom21gerbilsinfectedwiththefilarialparasiticwormBrugiapahangi.Lateralsaphenousveincountswereequivalenttoretroorbitalcountsatrelativelyhighcounts(greaterthan50microfilariaeper20#956;L)butweresignificantlylowerthanretroorbitalcountswhenmicrofilarialconcentrationswerelower.Ourresultsindicatethatalthoughretroorbitalcollectionmaybepreferablewhenlowconcentrationsofmicrofilariaeneedtobeenumerated,thelateralsaphenousveinisasuitablealternativesiteforbloodsamplingtodeterminemicrofilaremiaandisafeasiblerefinementthatcanbenefitthewellbeingofgerbils.

机译：TheNIHguidelinesforsurvivalbleedingofmiceandratsnotethatusingtheretroorbitalplexushasagreaterpotentialforcomplicationsthandoothermethodsofbloodcollectionandthatthisprocedureshouldbeperformedonanesthetizedanimals.Lateralsaphenousveinpuncturehasalowpotentialforcomplicationsandcanbeperformedwithoutanesthesia.Mongoliangerbils（Merionesunguiculatus）arethepreferredrodentmodelforfilarialparasiteresearch.Tomonitormicrofilariacountsintheblood，bloodsamplingfromtheorbitalplexushasbeenthestandard.Ourgoalwastorefinethebloodcollectiontechnique.Todeterminewhetherbloodcollectionfromthelateralsaphenousveinwasafeasiblealternativetoretroorbitalsampling，wecomparedmicrofilariacountsinbloodsamplescollectedbybothmethodsfrom21gerbilsinfectedwiththefilarialparasiticwormBrugiapahangi.Lateralsaphenousveincountswereequivalenttoretroorbitalcountsatrelativelyhighcounts（greaterthan50microfilariaeper20＃956; L）的Web TWE以不GNI达到ntlylo阅读rtha NRE甲硝唑LCO后打点ntswhe NMI CRO获得LCO NCE承包商的支持，我们nswe多项r.O的RRE不ltsi和tethata当将低浓度的丝虫精加到蜂巢中时，最好采用眶后收集，外侧隐静脉适合作为血样采样的替代部位，以确定微丝虫病和安全可行的提纯方法，以适应沙鼠的健康。

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《Comparative Medicine》 |2015年第6期|共页
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Alworth Leanne C; Berghaus Roy D; Kelly Lisa M; Supakorndej Prasit; Burkman Erica J; Savadelis Molly D; Cooper Tanya L; Salyards Gregory W; Harvey Stephen B; Moorhead Andrew R;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Assessment of Blood Collection from the Lateral Saphenous Vein for Microfilaria Counts in Mongolian Gerbils (Meriones unguiculatus) Infected with Brugia pahangi [J] . Alworth Leanne C., Berghaus Roy D., Kelly Lisa M., Comparative Medicine . 2015,第6期

机译：从大隐静脉感染的蒙古沙鼠（Meriones unguiculatus）的侧隐静脉收集的微丝aria计数的评估。
2. EFFECT OF IMMUNOSTIMULATORY OLIGODEOXYNUCLEOTIDES ON HOST RESPONSES AND THE ESTABLISHMENT OF BRUGIA PAHANGI IN MONGOLIAN GERBILS (MERIONES UNGUICULATUS) [J] . Sharon R. Chirgwin Jena M. Nowling Sharon U. Coleman and Thomas R. Klei* Journal of Parasitology . 2003,第3期

机译：免疫刺激性寡脱氧核糖核酸对蒙古沙鼠（MERIONES UNGUICULATUS）的寄主反应和建立布鲁日氏菌的影响
3. Removal of Wolbachia from Brugia pahangi Is Closely Linked to Worm Death and Fecundity but Does Not Result in Altered Lymphatic Lesion Formation in Mongolian Gerbils (Meriones unguiculatus) [J] . Sharon R. Chirgwin, Sharon U. Coleman, Kristina H. Porthouse, Infection and immunity . 2003,第12期

机译：从Bugia pahangi去除Wolbachia与蠕虫死亡和生殖力密切相关，但不会导致蒙古沙鼠（Meriones unguiculatus）淋巴病变形成改变
4. INTRAPERITONEAL INFECTIONS OF BRUGIA PAHANGI (NEMATODA: FILARIATA) IN MONGOLIAN JIRDS (MERIONES UNGUICULATUS): MICROFILARIAL POPULATION DYNAMICS AND HOST RESPONSE. [D] . TESTA, JACQUELINE ELIZABETH. 1980

机译：蒙古J（MERIONES UNGUICULATUS）中布鲁氏菌（线虫：FILARIATA）的腹膜内感染：微丝虫种群动态和寄主响应。
5. Assessment of Blood Collection from the Lateral Saphenous Vein for Microfilaria Counts in Mongolian Gerbils (Meriones unguiculatus) Infected with Brugia pahangi [O] . Leanne C Alworth, Roy D Berghaus, Lisa M Kelly, 2015

机译：从大隐静脉感染的蒙古沙鼠（Meriones unguiculatus）的侧隐静脉采集的血液中的微丝aria计数评估。
6. Removal of Wolbachia from Brugia pahangi Is Closely Linked to Worm Death and Fecundity but Does Not Result in Altered Lymphatic Lesion Formation in Mongolian Gerbils (Meriones unguiculatus) [O] . Chirgwin, Sharon R., Coleman, Sharon U., Porthouse, Kristina H., 2003

机译：从Bugia pahangi去除Wolbachia与蠕虫的死亡和生殖力密切相关，但不会导致蒙古沙鼠（Meriones unguiculatus）淋巴病变形成改变
7. Determination of 'Giardia lamblia' Cyst Infective Dose for the Mongolian Gerbil('Meriones unguiculatus') [R] . Schaefer, F. W., Johnson, C. H., Hsu, C. H., 1991

机译：测定蒙古沙鼠''meriones unguiculatus''的'贾第鞭毛虫'囊肿感染剂量

Assessment of Blood Collection from the Lateral Saphenous Vein for Microfilaria Counts in Mongolian Gerbils (Meriones unguiculatus) Infected with Brugia pahangi

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