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首页> 外文期刊>Comparative Medicine >Assessment of Blood Collection from the Lateral Saphenous Vein for Microfilaria Counts in Mongolian Gerbils (Meriones unguiculatus) Infected with Brugia pahangi
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Assessment of Blood Collection from the Lateral Saphenous Vein for Microfilaria Counts in Mongolian Gerbils (Meriones unguiculatus) Infected with Brugia pahangi

机译：从大隐静脉感染的蒙古沙鼠（Meriones unguiculatus）的侧隐静脉收集的微丝aria计数的评估。

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The NIH guidelines for survival bleeding of mice and rats note that using the retroorbital plexus has a greater potential for complications than do other methods of blood collection and that this procedure should be performed on anesthetized animals. Lateral saphenous vein puncture has a low potential for complications and can be performed without anesthesia. Mongolian gerbils (Meriones unguiculatus) are the preferred rodent model for filarial parasite research. To monitor microfilaria counts in the blood, blood sampling from the orbital plexus has been the standard. Our goal was to refine the blood collection technique. To determine whether blood collection from the lateral saphenous vein was a feasible alternative to retroorbital sampling, we compared microfilaria counts in blood samples collected by both methods from 21 gerbils infected with the filarial parasitic worm Brugia pahangi. Lateral saphenous vein counts were equivalent to retroorbital counts at relatively high counts (greater than 50 microfilariae per 20 mu L) but were significantly lower than retroorbital counts when microfilarial concentrations were lower. Our results indicate that although retroorbital collection may be preferable when low concentrations of microfilariae need to be enumerated, the lateral saphenous vein is a suitable alternative site for blood sampling to determine microfilaremia and is a feasible refinement that can benefit the wellbeing of gerbils.
机译：NIH关于小鼠和大鼠存活出血的指南指出，与其他采血方法相比，使用眶后神经丛具有更大的并发症发生可能性，并且该程序应在麻醉的动物上进行。隐性大隐静脉穿刺的并发症发生率较低，无需麻醉即可进行。蒙古沙鼠（Meriones unguiculatus）是丝虫寄生虫研究的首选啮齿动物模型。为了监测血液中的微丝aria计数，从眼眶神经丛采血已成为标准。我们的目标是完善采血技术。为了确定从大隐静脉外侧收集血液是否可以替代眶后采样，我们比较了两种方法收集的从感染了丝虫寄生虫布鲁贾氏菌的21只沙鼠中收集的血液样品中的微丝aria计数。大隐静脉的外侧计数在较高计数时相当于眶后计数（每20μL大于50个微丝aria），但在微丝浓度较低时显着低于眶后计数。我们的结果表明，尽管当需要枚举低浓度的微丝aria时，眶后收集可能是更可取的，但隐隐外侧静脉是血液采样以确定微丝ar病的合适替代部位，并且是可行的改良方法，可以使沙鼠的健康受益。

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