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首页> 外文期刊>Comparative and functional genomics >Comparison of Metabolic Network between Muscle and Intramuscular Adipose Tissues in Hanwoo Beef Cattle Using a Systems Biology Approach
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Comparison of Metabolic Network between Muscle and Intramuscular Adipose Tissues in Hanwoo Beef Cattle Using a Systems Biology Approach

机译：用系统生物学方法比较韩宇牛肉牛肌肉和肌肉内脂肪组织之间的代谢网络

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The interrelationship between muscle and adipose tissues plays a major role in determining the quality of carcass traits. The objective of this study was to compare metabolic differences between muscle and intramuscular adipose (IMA) tissues in the longissimus dorsi (LD) of Hanwoo (Bos taurus coreanae) using the RNA-seq technology and a systems biology approach. The LD sections between the 6th and 7th ribs were removed from nine (each of three cows, steers, and bulls) Hanwoo beef cattle (carcass weight of  kg) immediately after slaughter. The total mRNA from muscle, IMA, and subcutaneous adipose and omental adipose tissues were isolated and sequenced. The reads that passed quality control were mapped onto the bovine reference genome (build bosTau6), and differentially expressed genes across tissues were identified. The KEGG pathway enrichment tests revealed the opposite direction of metabolic regulation between muscle and IMA. Metabolic gene network analysis clearly indicated that oxidative metabolism was upregulated in muscle and downregulated in IMA. Interestingly, pathways for regulating cell adhesion, structure, and integrity and chemokine signaling pathway were upregulated in IMA and downregulated in muscle. It is thus inferred that IMA may play an important role in the regulation of development and structure of the LD tissues and muscle/adipose communication.
机译：肌肉和脂肪组织之间的相互关系在决定car体性状的质量方面起着重要作用。这项研究的目的是使用RNA序列技术和系统生物学方法，比较Hanwoo（Bos taurus coreanae）背最长肌（LD）的肌肉和肌内脂肪（IMA）组织之间的代谢差异。屠宰后立即从九头（三头牛，ste牛和公牛中的每头）Hanwoo肉牛（car体重为kg）中去除了第六和第七肋之间的LD区域。分离并测序了来自肌肉，IMA以及皮下脂肪和网膜脂肪组织的总mRNA。通过质量控制的读数被定位到牛参考基因组（构建体bosTau6）上，并鉴定出跨组织的差异表达基因。 KEGG途径富集试验揭示了肌肉与IMA之间代谢调节的相反方向。代谢基因网络分析清楚地表明，氧化代谢在肌肉中上调而在IMA中下调。有趣的是，IMA中上调细胞黏附，结构和完整性的途径以及趋化因子信号通路，而肌肉中下调。因此推断IMA在调节LD组织的发育和结构以及肌肉/脂肪通讯中可能起重要作用。

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