Alterations in Methylation and Expression Levels of Imprinted Genes H19 and Igf2 in the Fetuses of Diabetic Mice

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Alterations in Methylation and Expression Levels of Imprinted Genes H19 and Igf2 in the Fetuses of Diabetic Mice

机译：糖尿病小鼠胎儿甲基化和印迹基因H19和Igf2表达水平的变化

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Thestudyaimedtorevealalterationsinexpressionandmethylationlevelsofthegrowth-relatedimprintedgenesH19andIgf2infetusesofdiabeticmice.Diabeteswasinducedinfemalemicebyintraperitonealinjectionofstreptozotocin.DNAandtotalRNAwereextractedfromfetusesobtainedfromdiabeticandcontroldamsonembryonicday(E)14.Real-timeRT-PCRanalysisrevealedthatthemRNAexpressionofIgf2infetusesfromdiabeticmicewas0.65-foldofthecontrolcounterparts.BisulfitegenomicsequencingdemonstratedthatthemethylationleveloftheH19#8211;Igf2imprintcontrolregionwas19.1%higherindiabeticfetusesthaninthoseofcontroldams.Inaddition,thebodyweightofpupsborntodiabeticdamswas26.5%lowerthanthatofthecontrolgroup.Theresultsindicatethatmaternaldiabetescanaffectfetaldevelopmentbymeansofalteredexpressionofimprintedgenes.ThemodifiedgenomicDNAmethylationstatusofimprintinggenesmayaccountforthechangeingeneexpression.

机译：Thestudyaimedtorevealalterationsinexpressionandmethylationlevelsofthegrowth-relatedimprintedgenesH19andIgf2infetusesofdiabeticmice.Diabeteswasinducedinfemalemicebyintraperitonealinjectionofstreptozotocin.DNAandtotalRNAwereextractedfromfetusesobtainedfromdiabeticandcontroldamsonembryonicday（E）14.Real-timeRT-PCRanalysisrevealedthatthemRNAexpressionofIgf2infetusesfromdiabeticmicewas0.65-foldofthecontrolcounterparts.BisulfitegenomicsequencingdemonstratedthatthemethylationleveloftheH19＃8211; Igf2imprintcontrolregionwas19.1％higherindiabeticfetusesthaninthoseofcontroldams.Inaddition，thebodyweightofpupsborntodiabeticdamswas26.5％lowerthanthatofthecontrolgroup.Theresultsindicatethatmaternaldiabetescanaffectfetaldevelopmentbymeansofalteredexpressionofimprintedgenes.ThemodifiedgenomicDNAmethylationstatusofimprintinggenesmayaccountforthechangeingeneexpression。

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《Comparative Medicine》 |2008年第4期|共页
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Alterations in methylation and expression levels of imprinted genes H19 and Igf2 in the fetuses of diabetic mice. [J] . Shao WJ, Tao LY, Gao C, Comparative Medicine . 2008,第4期

机译：糖尿病小鼠胎儿甲基化和印迹基因H19和Igf2表达水平的变化。
2. Homocysteine harasses the imprinting expression of IGF2 and H19 by demethylation of differentially methylated region between IGF2/H19 genes [J] . Lijuan Li Jing Xie Meng Zhang and Shuren Wang* Acta Biochimica et Biophysica Sinica . 2009,第6期

机译：同型半胱氨酸通过IGF2 / H19基因之间差异甲基化区域的去甲基化来破坏IGF2和H19的印迹表达
3. Homocysteine harasses the imprinting expression of IGF2 and H19 by demethylation of differentially methylated region between IGF2/H19 genes [J] . Lijuan Li, Jing Xie, Meng Zhang, 生物化学与生物物理学报：英文版 . 2009,第006期

机译：同型半胱氨酸通过IGF2 / H19基因之间的甲基化区域甲基化来破坏IGF2和H19的印迹表达
4. TRANSIENT EXPRESSION OF GENES WITH ALTERED METHYLATION LEVELS IN TOBACCO PROTOPLASTS [C] . N. BUCHERNA, L.E. HESZKY, I. NAGY Induced mutations and molecular techniques for crop improvement . 1995

机译：甲基化水平改变的基因在烟草原生质体中的瞬时表达
5. Transgenic RNAi reveals essential functions for CTCF in H19/IGF2 imprinting and preimplantation development. [D] . Fedoriw, Andrew. 2004

机译：转基因RNAi揭示了CTCF在H19 / IGF2印迹和植入前发育中的基本功能。
6. Alterations in Methylation and Expression Levels of Imprinted Genes H19 and Igf2 in the Fetuses of Diabetic Mice [O] . Wei-Juan Shao, Ling-Yun Tao, Cheng Gao, 2008

机译：糖尿病小鼠胎儿甲基化和印迹基因H19和Igf2表达水平的变化
7. Homocysteine harasses the imprinting expression of IGF2 and H19 by demethylation of differentially methylated region between IGF2/H19 genes [O] . Lijuan Li, Jing Xie, Meng Zhang, 2015

机译：同型半胱氨酸通过IGF2 / H19基因之间差异甲基化区域的去甲基化来骚扰IGF2和H19的印迹表达
8. Modeling Human Epithelial Ovarian Cancer in Mice by Alteration of Expression of the Expression of the BRCA1 and/p53 Genes [R] . Connolly, D. C. 2006

机译：通过改变BRCa1和/ p53基因表达对小鼠人卵巢上皮性癌的建模

Alterations in Methylation and Expression Levels of Imprinted Genes H19 and Igf2 in the Fetuses of Diabetic Mice

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