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首页> 外文期刊>Comparative Medicine >Alterations in methylation and expression levels of imprinted genes H19 and Igf2 in the fetuses of diabetic mice.
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Alterations in methylation and expression levels of imprinted genes H19 and Igf2 in the fetuses of diabetic mice.

机译：糖尿病小鼠胎儿甲基化和印迹基因H19和Igf2表达水平的变化。

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The study aimed to reveal alterations in expression and methylation levels of the growth-related imprinted genes H19 and Igf2 in fetuses of diabetic mice. Diabetes was induced in female mice by intraperitoneal injection of streptozotocin. DNA and total RNA were extracted from fetuses obtained from diabetic and control dams on embryonic day (E) 14. Real-time RT-PCR analysis revealed that the mRNA expression of Igf2 in fetuses from diabetic mice was 0.65-fold of the control counterparts. Bisulfite genomic sequencing demonstrated that the methylation level of the H19-Igf2 imprint control region was 19.1% higher in diabetic fetuses than in those of control dams. In addition, the body weight of pups born to diabetic dams was 26.5% lower than that of the control group. The results indicate that maternal diabetes can affect fetal development by means of altered expression of imprinted genes. The modified genomic DNA methylation status of imprinting genes may account for the change in gene expression.
机译：该研究旨在揭示糖尿病小鼠胎儿中与生长相关的印迹基因H19和Igf2的表达和甲基化水平的变化。通过腹膜内注射链脲佐菌素在雌性小鼠中诱发糖尿病。在胚胎第14天，从糖尿病和对照大坝获得的胎儿中提取DNA和总RNA。实时RT-PCR分析显示，糖尿病小鼠的胎儿中Igf2的mRNA表达是对照小鼠的0.65倍。亚硫酸氢盐基因组测序表明，糖尿病胎儿中H19-Igf2印迹控制区域的甲基化水平比对照大坝高19.1％。此外，糖尿病大坝出生的幼犬的体重比对照组低26.5％。结果表明，母亲糖尿病可以通过改变印迹基因的表达来影响胎儿的发育。印迹基因的修饰的基因组DNA甲基化状态可以解释基因表达的变化。

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