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首页> 外文期刊>Biology Open >Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in?Arabidopsis thaliana
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Expression of Cucumber mosaic virus suppressor 2b alters FWA methylation and its siRNA accumulation in?Arabidopsis thaliana

机译：黄瓜花叶病毒抑制剂2b的表达改变拟南芥FWA甲基化及其siRNA的积累

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The Cucumber mosaic virus (CMV) suppressor 2b co-localizes with AGO4 in cytoplasmic and nuclear fractions of?Arabidopsis thaliana. Biochemical fractionation of?A. thaliana?cellular extracts revealed that 2b and AGO4 coexist in multiple size exclusions. 2b transgenic?A. thaliana?exhibited an enhanced accumulation of 24nt siRNAs from flowering wageningen (FWA) and other heterochromatic loci. These plants also exhibited hypo-methylation of an endogenous- as well as transgene-FWA promoter at non-CG sites. In corroboration, both transgenic 2b and CMV infection affected the regulation of transposons which mimics the?ago4?phenotype. In conclusion, 2b perturbs plant defense by interfering with AGO4-regulated transcriptional gene silencing.
机译：黄瓜花叶病毒（CMV）抑制子2b与AGO4在拟南芥的胞质和核级分中共定位。 A的生化分级。拟南芥细胞提取物显示2b和AGO4共存于多种大小的排斥中。 2b转基因？拟南芥（Thaliana）展示了来自开花花宁宁（FWA）和其他异色基因座的24nt siRNA的增强积累。这些植物在非CG位点还表现出内源性和转基因-FWA启动子的低甲基化。确证的是，转基因2b和CMV感染都影响转座子的调控，该转座子模仿ago4α表型。总之，2b通过干扰AGO4调控的转录基因沉默来干扰植物防御。

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