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Culture Medium Optimization for Production of Rhamnolipids by Burkholderia glumae

机译：伯克霍尔德氏菌生产鼠李糖脂的培养基优化

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Burkholderia glumae is a biosafety level 1 bacterium capable of producing rhamnolipid biosurfactant with longer 3-hydroxy fatty acid chains moieties than those produced by the prototypal producer, the opportunistic pathogen Pseudomonas aeruginosa. Although the capacity of production of rhamnolipid, and the parameters affecting this production, are well established for P. aeruginosa, little is known about the factors that may affect their production in B. glumae. Hence, to evaluate and enhance the production of rhamnolipids in B. glumae, following the selection of best carbon and nitrogen sources, a two-level fractional factorial design experiment was performed to identify the limiting factors significantly affecting the production of rhamnolipids in this bacterial species. Effects of six inorganic nutrients and two physical parameters were studied, and mannitol, urea, CaCl2, and potassium phosphate buffer were selected for further optimization by applying a response surface methodology (RSM). Under the identified optimized conditions, a rhamnolipid production of 1.66 g/L was obtained, about five times higher than that of the initial non-optimized conditions. This represents a key step in the development of large-scale production processes.
机译：伯克霍尔德氏菌是一种生物安全等级为1的细菌，能够产生鼠李糖脂生物表面活性剂，其3-羟基脂肪酸链部分比原型生产者，机会病原体铜绿假单胞菌产生的鼠李糖生物表面活性剂更长。尽管鼠李糖脂的生产能力和影响该产量的参数已为铜绿假单胞菌充分确立，但对影响其在小球藻中生产的因素知之甚少。因此，为了评估和增强小生芽孢杆菌中鼠李糖脂的产生，在选择了最佳碳源和氮源之后，进行了两级分数阶乘设计实验，以鉴定显着影响该细菌种鼠李糖脂产生的限制因素。研究了六种无机营养素和两个物理参数的影响，并通过应用响应面方法（RSM）选择了甘露醇，尿素，CaCl2和磷酸钾缓冲液进行进一步优化。在确定的优化条件下，鼠李糖脂产量为1.66 g / L，比初始非优化条件高约5倍。这代表着大规模生产工艺发展的关键一步。

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