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首页> 外文期刊>Comparative and functional genomics >Transcriptome Analysis Reveals Dynamic Fat Accumulation in the Walnut Kernel
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Transcriptome Analysis Reveals Dynamic Fat Accumulation in the Walnut Kernel

机译：转录组分析揭示了核桃仁中的动态脂肪积累

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Walnut (Juglans regia L.) is an important woody oilseed species cultivated throughout the world. In this study, comparative transcript profiling was performed using high-throughput RNA sequencing technology at the following three stages of walnut fat synthesis in the “Lvling” walnut cultivar the initial developmental stage (L1), the fast developing stage (L2), and the last developing stage (L3). A total of 68.18 GB of data were obtained on the three developmental stages, and 92% to 94% of clean data were able to be located to the reference genome. Further comparisons of the transcripts in the three libraries revealed that 724, 2027, and 4817 genes were differentially expressed between the L2 and L1 (L2vsL1), L3 and L2 (L3vsL2), and L3 and L1 (L3vsL1) samples, respectively. Through the GO gene enrichment analysis, differentially expressed genes (DEGs) in L2vsL1, L3vsL2, and L3vsL1 were enriched into 3, 0, and 2 functional categories, respectively. According to the KEGG enrichment analysis, DEGs in L2vsL1, L3vsL2, and L3vsL1 were annotated into 77, 110, and 3717 taxonomic metabolic pathways in the KEGG database, respectively. Next, we analyzed expression levels of genes related to fat synthesis. Our results indicated that ACCase, LACS, and FAD7 were the key genes related to fat synthesis. The high-throughput transcriptome sequencing of walnut in different developmental stages has greatly enriched the current genomic available resources. The comparison of DEGs under different developmental stages identified a wealth of candidate genes involved in fat synthesis, which will facilitate further genetic improvement and molecular studies of the walnut.
机译：核桃（Juglans regia L.）是全世界种植的重要木本油料树种。在本研究中，使用“高通量” RNA测序技术，在“ Lvling”核桃品种的核桃脂肪合成的以下三个阶段（初始发育阶段（L1），快速发育阶段（L2）和最后开发阶段（L3）。在三个发育阶段总共获得了68.18 GB的数据，并且92％至94％的干净数据能够定位到参考基因组。进一步比较三个库中的转录本后发现，分别在L2和L1（L2vsL1），L3和L2（L3vsL2），L3和L1（L3vsL1）样品之间差异表达了724、2027和4817个基因。通过GO基因富集分析，L2vsL1，L3vsL2和L3vsL1中的差异表达基因（DEG）分别分为3、0和2个功能类别。根据KEGG富集分析，在KEGG数据库中，L2vsL1，L3vsL2和L3vsL1中的DEG分别注释为77、110和3717个分类代谢途径。接下来，我们分析了与脂肪合成相关的基因的表达水平。我们的结果表明，ACCase，LACS和FAD7是与脂肪合成相关的关键基因。核桃在不同发育阶段的高通量转录组测序极大地丰富了当前的基因组可用资源。通过比较不同发育阶段的DEG，可以确定大量参与脂肪合成的候选基因，这将有助于核桃的进一步遗传改良和分子研究。

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