Intracytoplasmic Sperm Injection (ICSI) Enables Rescue of Valuable Mutant Mouse Strains

Li Ming-Wen; McGinnis Lynda; Zhu Liben; Lawitts Joel; Biggers John; Lloyd K. C. Kent

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Intracytoplasmic Sperm Injection (ICSI) Enables Rescue of Valuable Mutant Mouse Strains

机译：胞浆内精子注射（ICSI）可以挽救宝贵的突变小鼠品系

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Geneticallyalteredmiceareimportantresearchtoolsforthestudyofhumandevelopmentanddisease.Occasionally,whetherornotrelatedtothegeneticmutation,micemaybecomeinfertilewithageand,thus,risklossofthemutantline.Underconditionsinwhichassistedreproductiontechniques(ARTs),suchasinvitrofertilization,areunsuccessful,anewstrategy,intracytoplasmicsperminjection(ICSI),maybeapplicable.Thistechniquehasbeenperfectedforuseinthemouseandisnowconsideredareliable,effective,andefficientART.Inthestudyreportedhere,we#8220;rescued#8221;(i.e.,producedoffspring,usingICSIfroma#8220;last-of-line#8221;mutantmalemouse)fourlinesthatotherwisehadbecomeinfertileandunresponsivetoconventionalART's.Atotalof26livepupswereproducedfromeightpregnantrecipientfostermothers.Fivemutantmalemicewerederived(oneeachfromthreelines,andtwofromoneline),andallsurvivedtoadulthood.WefoundthatlivebornmicecouldbesuccessfullyderivedbyuseofICSIthatsubsequentlycouldbreedbynaturalmatingtoreestablishthemutantline.Becauseofitseffectivenessandreliabilityundertheseconditions,ICSIshouldbeconsideredapowerfuladditiontothearmamentariumofART'sapplicableinthegenetically-alteredmouse,especiallywhenonlyonemalemaystillbeavailable.

机译：Geneticallyalteredmiceareimportantresearchtoolsforthestudyofhumandevelopmentanddisease.Occasionally，whetherornotrelatedtothegeneticmutation，micemaybecomeinfertilewithageand，从而，risklossofthemutantline.Underconditionsinwhichassistedreproductiontechniques（艺术），suchasinvitrofertilization，areunsuccessful，anewstrategy，intracytoplasmicsperminjection（ICSI），maybeapplicable.Thistechniquehasbeenperfectedforuseinthemouseandisnowconsideredareliable，有效，andefficientART.Inthestudyreportedhere，我们＃8220;救出＃8221;（即，producedoffspring ，usingICSIfroma＃8220;最后的行＃8221; mutantmalemouse）fourlinesthatotherwisehadbecomeinfertileandunresponsivetoconventionalART's.Atotalof26livepupswereproducedfromeightpregnantrecipientfostermothers.Fivemutantmalemicewerederived（oneeachfromthreelines，andtwofromoneline），andallsurvivedtoadulthood.WefoundthatlivebornmicecouldbesuccessfullyderivedbyuseofICSIthatsubsequentlycouldbreedbynaturalmatingtoreestablishthemutantline.Becauseofitseffectivenessandreliability在这种情况下，ICS应考虑向基因改变的小鼠，特别是在只有一名男性仍然可用的情况下，向ART的军备库中添加一个强大的功能。

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《Comparative Medicine》 |2003年第3期|共页
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Li Ming-Wen; McGinnis Lynda; Zhu Liben; Lawitts Joel; Biggers John; Lloyd K. C. Kent;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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1. Intracytoplasmic sperm injection (ICSI) enables rescue of valuable mutant mouse strains [J] . Li MW, McGinnis L, Zhu LB, Comparative Medicine . 2003,第3期

机译：胞浆内精子注射（ICSI）可挽救有价值的突变小鼠品系
2. FACS selection of valuable mutant mouse round spermatids and strain rescue via round spermatid injection [J] . Zhu Lian, Zhou Wei, Kong Peng-Cheng, Zygote . 2015,第3期

机译：FACS筛选有价值的突变小鼠圆形精子并通过圆形精子注射拯救菌株
3. Development Ability of Vitrified Mouse Oocytes Assessed by Two Fertilization Methods: Intracytoplasmic Sperm Injection (ICSI) and Laser Assisted IVF [J] . Claudia CARSTEA, Alexandru T. BOGDAN, Judith IPATE, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies . 2013,第1a2期

机译：两种受精方法评估的玻璃化小鼠卵母细胞的发育能力：胞浆内精子注射（ICSI）和激光辅助IVF
4. A Comparison of Pregnancy Rates in Female Partners of Males with Sperm Laden with Antisperm Antibodies Following Intrauterine Insemination (IUI) with Sperm Treated with Chymotrypsin Galactose vs. In Vitro Fertilization (IVF) with Intracytoplasmic Sperm Injection (ICSI) [C] . A. Bollendorf, J.H. Check, C. Wilson International Congress of Andrology . 2009

机译：用宫内生精（IUI）与宫内节制物（IUI）用胰蛋白酶蛋白酶蛋白酶蛋白酶术（IVF）对宫内节制物（IUI）患有抗血糖抗体的妊娠抗体与抗血清抗体的妊娠率比较。（ICSI）
5. Robotic Cell Manipulation for Intracytoplasmic Sperm Injection (ICSI) [D] . Dai, Changsheng. 2020

机译：氏菌精子注射的机器人细胞操作（ICSI）
6. Perinatal and neonatal outcomes of pregnancies after early rescue intracytoplasmic sperm injection in women with primary infertility compared with conventional intracytoplasmic sperm injection: a retrospective 6-year study [O] . Feng Xiong, Qing Sun, Guangui Li, 2020

机译：早期抢救后怀孕后的围产期和新生儿结果与常规患有常规血管科学精子注射的妇女患有初级不孕症的妇女：回顾性为6年的研究
7. Near futility of rescue intracytoplasmic sperm injection (ICSI) and the advantage of vitrification over fresh transfer [O] . B. Slocum, K.S. Richter, M.J. Tucker, 2018

机译：抢救难以徒劳无功的抢救患者精子注射（ICSI）和玻璃化对新鲜转移的优势

Intracytoplasmic Sperm Injection (ICSI) Enables Rescue of Valuable Mutant Mouse Strains

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