• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Comparative Medicine >Intracytoplasmic sperm injection (ICSI) enables rescue of valuable mutant mouse strains
【24h】

Intracytoplasmic sperm injection (ICSI) enables rescue of valuable mutant mouse strains

机译：胞浆内精子注射（ICSI）可挽救有价值的突变小鼠品系

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Genetically altered mice are important research tools for the study of human development and disease. Occasionally, whether or not related to the genetic mutation, mice may become infertile with age and, thus, risk loss of the mutant line. Under conditions in which assisted reproduction techniques (ARTs), such as in vitro fertilization, are unsuccessful, a new strategy, intracytoplasmic sperm injection (ICSI), may be applicable. This technique has been perfected for use in the mouse and is now considered a reliable, effective, and efficient ART. In the study reported here, we "rescued" (i.e., produced offspring, using ICSI from a "last-of-line" mutant male mouse) four lines that otherwise had become infertile and unresponsive to conventional ART's. A total of 26 live pups were produced from eight pregnant recipient foster mothers. Five mutant male mice were derived (one each from three lines, and two from one line), and all survived to adulthood. We found that live born mice could be successfully derived by use of ICSI that subsequently could breed by natural mating to reestablish the mutant line. Because of its effectiveness and reliability under these conditions, ICSI should be considered a powerful addition to the armamentarium. of ART's applicable in the genetically-altered mouse, especially when only one male may still be available.
机译：转基因小鼠是研究人类发育和疾病的重要研究工具。有时，无论是否与基因突变相关，小鼠都可能随着年龄的增长而变得不育，因此，可能会丧失突变株的风险。在辅助生殖技术（ART）（例如体外受精）不成功的情况下，可以采用一种新的策略，即胞浆内精子注射（ICSI）。此技术已完美用于鼠标，现在被认为是可靠，有效和高效的ART。在此处报道的研究中，我们“拯救”了四个品系（即使用来自“后代”突变型雄性小鼠的ICSI产生的后代）四个本来已经不育且对常规ART无反应的品系。从八名怀孕的寄养母亲那里生产了总共26只活仔。衍生出五只突变的雄性小鼠（每只来自三系，两只来自一系），并且全部存活到成年。我们发现活体出生的小鼠可以通过使用ICSI成功获得，随后可以通过自然交配繁殖以重建突变体系。由于在这样的条件下它的有效性和可靠性，ICSI应该被认为是军备库的强大补充。 ART的方法适用于基因改变的小鼠，特别是当只有一只雄性仍然可用时。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号