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首页> 外文期刊>Comparative and functional genomics >Changes in miRNA Gene Expression during Wound Repair in Differentiated Normal Human Bronchial Epithelium
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Changes in miRNA Gene Expression during Wound Repair in Differentiated Normal Human Bronchial Epithelium

机译：分化正常人支气管上皮伤口修复过程中miRNA基因表达的变化

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Purpose. Airway epithelium acts as a protective barrier against the particles from the inhaled air. Damage to the epithelium may result in loss of the barrier function. Epithelial repair in response to injury requires complex mechanisms, such as microRNA, small noncoding molecules, to regulate the processes involved in wound repair. We aimed to establish if the microRNA gene expression profile is altered during the airway epithelial repair in differentiated cells. Methods. miRNA gene expression profile during the wound closure of differentiated normal human bronchial epithelium (NHBE) from one donor was analysed using quantitative real-time PCR. We have analysed the expression of 754 genes at five time points during a 48-hour period of epithelium repair using TaqMan Low Density Array. Results. We found out that 233 miRNA genes were expressed in normal human bronchial epithelium. Twenty miRNAs were differentially expressed during the wound repair process, but only one (miR-455-3p) showed significance after FDR adjustment (). Using STEM, we have identified two clusters of several miRNA genes with similar expression profile. Pathway enrichment analysis showed several significant signaling pathways altered during repair, mainly involved in cell cycle regulation, proliferation, migration, adhesion, and transcription regulation. Conclusions. miRNA expression profile is altered during airway epithelial repair of differentiated cells from one donor in response to mechanical injury in vitro, suggesting their potential role in wound repair.
机译：目的。气道上皮起到了抵抗吸入空气中颗粒物的保护性屏障的作用。上皮损伤可能导致屏障功能丧失。响应损伤的上皮修复需要复杂的机制（例如microRNA，小的非编码分子）来调节涉及伤口修复的过程。我们旨在确定在分化细胞的气道上皮修复过程中，microRNA基因表达谱是否发生改变。方法。使用定量实时PCR分析来自一位供体的分化正常人支气管上皮（NHBE）伤口闭合过程中的miRNA基因表达谱。我们已经使用TaqMan低密度阵列在上皮修复的48小时内的五个时间点分析了754个基因的表达。结果。我们发现正常人支气管上皮中表达了233个miRNA基因。在伤口修复过程中有20种miRNA差异表达，但在FDR调整后只有一种（miR-455-3p）显示出重要意义。使用STEM，我们已经鉴定了具有相似表达谱的几个miRNA基因的两个簇。途径富集分析显示，修复过程中改变了几个重要的信号通路，主要涉及细胞周期调控，增殖，迁移，粘附和转录调控。结论。 miRNA的表达谱在气道上皮修复一个供体的分化细胞过程中发生了变化，以响应体外的机械损伤，表明它们在伤口修复中的潜在作用。

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