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首页> 外文期刊>Comparative and functional genomics >Transposable Elements in the Organization and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)
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Transposable Elements in the Organization and Diversification of the Genome of Aegilops speltoides Tausch (Poaceae, Triticeae)

机译：羊草（Aegilops speltoides Tausch）（禾本科，黑麦）的基因组的组织和多样化中的转座因子

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Repetitive DNA—specifically, transposable elements (TEs)—is a prevailing genomic fraction in cereals that underlies extensive genome reshuffling and intraspecific diversification in the wild. Although large amounts of data have been accumulated, the effect of TEs on the genome architecture and functioning is not fully understood. Here, plant genome organization was addressed by means of cloning and sequencing TE fragments of different types, which compose the largest portion of the Aegilops speltoides genome. Individual genotypes were analyzed cytogenetically using the cloned TE fragments as the DNA probes for fluorescence in situ hybridization (FISH). The obtained TE sequences of the Ty1-copia, Ty3-gypsy, LINE, and CACTA superfamilies showed the relatedness of the Ae. speltoides genome to the Triticeae tribe and similarities to evolutionarily distant species. A significant number of clones consisted of intercalated fragments of TEs of various types, in which Fatima (Ty3-gypsy) sequences predominated. At the chromosomal level, different TE clones demonstrated sequence-specific patterning, emphasizing the effect of the TE fraction on the Ae. speltoides genome architecture and intraspecific diversification. Altogether, the obtained data highlight the current species-specific organization and patterning of the mobile element fraction and point to ancient evolutionary events in the genome of Ae. speltoides.
机译：重复性DNA，特别是转座因子（TEs），是谷物中占主导地位的基因组部分，是野生环境中广泛的基因组改组和种内多样化的基础。尽管已经积累了大量数据，但对TEs对基因组结构和功能的影响尚不完全清楚。在这里，通过克隆和测序不同类型的TE片段来解决植物基因组的组织问题，这些片段构成了Aegilops speltoides基因组的最大部分。使用克隆的TE片段作为荧光原位杂交（FISH）的DNA探针，通过细胞遗传学分析了单个基因型。 Ty1-copia，Ty3-gypsy，LINE和CACTA超家族的TE序列显示出Ae的相关性。将小麦的speltoides基因组与黑麦部落相联系，并与进化上较远的物种相似。大量克隆由各种类型的TE的插入片段组成，其中以法蒂玛（Ty3-gypsy）序列为主。在染色体水平上，不同的TE克隆表现出序列特异性模式，强调了TE组分对Ae的影响。 speltoides基因组结构和种内多样性。总而言之，获得的数据突出了当前的特定物种的组织和活动元素部分的模式，并指出了Ae基因组中的古代进化事件。 speltoides。

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