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首页> 外文期刊>Biology Open >High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment
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High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment

机译：通过直接红细胞裂解处理高产分离经血来源的子宫内膜干细胞

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Recently, menstrual blood-derived endometrial stem cells (MenSCs) have become attractive for stem cell based therapy due to their abundance, easy and non-invasive extraction and isolation process, high proliferative capacity, and multi-lineage differentiation potential. MenSC-based therapies for various diseases are being extensively researched. However, the high death rate and poor engraftment in sites of damaged tissues reduce the therapeutic value of these stem cells for transplantation. In theory, periodic stem cell transplantation is an alternative strategy to overcome the challenge of the loss of beneficial stem cell-derived effects due to the rapid disappearance of the stem cellsin vivo. However, periodic stem cell transplantation requires sufficient amounts of the desired stem cells with a low number of subculture passages. Our previous results have demonstrated that primary MenSCs mainly reside in the deciduous endometrium, and considerable amounts of deciduous endometrium intertwined with menstrual blood clots were discarded after conventional density gradient centrifugation (DGC). Therefore, the aim of this study was to determine whether primary MenSCs exist in the sedimentation of the deciduous endometrium after DGC and further to evaluate the isolation of MenSCs by direct red blood cell lysis treatment. As expected, our results confirmed that substantial amounts of primary MenSCs still remain in the sedimentation after DGC and indicated that MenSC isolation by directly lysing the red blood cells not only guaranteed substantial amounts of superior MenSCs with a low number of subculture passages, but also was time efficient and economical, providing a solid support for extensive clinical application.
机译：近来，由于经血来源丰富的子宫内膜干细胞（MenSCs）丰富，易于和非侵入性的提取和分离过程，高增殖能力和多系分化潜力，它们已成为基于干细胞治疗的诱人方法。基于MenSC的各种疾病的疗法正在被广泛研究。然而，高死亡率和受损组织部位的不良移植降低了这些干细胞的移植治疗价值。从理论上讲，定期的干细胞移植是一种替代策略，可以克服由于干细胞在体内迅速消失而丧失有益的干细胞衍生效应的挑战。但是，定期干细胞移植需要足够数量的所需干细胞，且传代次数较少。我们以前的结果表明，原发性MenSCs主要存在于落叶子宫内膜，常规密度梯度离心（DGC）后丢弃了大量与月经血凝结在一起的落叶子宫内膜。因此，本研究的目的是确定DGC后落叶子宫内膜的沉积物中是否存在原发性MenSCs，并进一步评估通过直接红细胞裂解处理对MenSCs的分离。正如预期的那样，我们的结果证实了DGC后沉淀物中仍然保留了大量初级MenSC，并表明通过直接裂解红细胞分离MenSC不仅可以确保大量次代传代次数少的优质MenSC，而且还可以省时又经济，为广泛的临床应用提供了坚实的支持。

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