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Genotype-dependent Burst of Transposable Element Expression in Crowns of Hexaploid Wheat (Triticum aestivum L.) during Cold Acclimation

机译:基因型依赖的六倍体小麦(Triticum aestivum L.)顶冠冷转化过程中转座子表达的爆发

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The expression of 1,613 transposable elements (TEs) represented in the Affymetrix Wheat Genome Chip was examined during cold treatment in crowns of four hexaploid wheat genotypes that vary in tolerance to cold and in flowering time. The TE expression profiles showed a constant level of expression throughout the experiment in three of the genotypes. In winter Norstar, the most cold-hardy of the four genotypes, a subset of the TEs showed a burst of expression after vernalization saturation was achieved. About 47% of the TEs were expressed, and both Class I (retrotransposons) and Class II (DNA transposons) types were well represented.GypsyandCopiawere the most represented among the retrotransposons whileCACTAandMarinerwere the most represented DNA transposons. The data suggests that theVrn-A1region plays a role in the stage-specific induction of TE expression in this genotype.
机译:在冷处理期间,对四种六倍体小麦基因型的冠部进行冷处理期间,检查了Affymetrix小麦基因组芯片中代表的1,613个可转座元件(TEs)的表达,这些基因型在耐寒性和开花时间方面有所不同。在整个实验中,TE的表达谱在三种基因型中均表现出恒定的表达水平。在冬季Norstar(四种基因型中最耐寒的)中,TE的一个子集在达到春化饱和后显示出一阵表达。大约47%的TE表达,并且I类(逆转座子)和II类(DNA转座子)类型都得到了很好的表现,吉普赛和科皮亚在逆转座子中代表最多,而CACTA和Mariner是最代表的DNA转座子。数据表明,在该基因型中,Vrn-A1区域在TE表达的阶段特异性诱导中起作用。

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