...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Quantitative expression analysis of selected COR genes reveals their differential expression in leaf and crown tissues of wheat (Triticum aestivum L.) during an extended low temperature acclimation regimen.
【24h】

Quantitative expression analysis of selected COR genes reveals their differential expression in leaf and crown tissues of wheat (Triticum aestivum L.) during an extended low temperature acclimation regimen.

机译：选定的COR基因的定量表达分析揭示了它们在延长的低温驯化过程中在小麦（小麦）的叶片和冠状组织中的差异表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A number of COR genes (COld-Regulated genes) have been implicated in the acquisition of low temperature (LT) tolerance in wheat (Triticum aestivum L.). This study compared the relative expression patterns of selected COR genes in leaf and crown tissues of wheat near-isogenic lines to increase understanding of the molecular mechanisms underlying LT acclimation. Reciprocal near-isogenic lines were generated such that the dominant Vrn-A1 and recessive vrn-A1 loci were interchanged in a spring cv. Manitou and a winter cv. Norstar. Phenological development, acquisition of LT tolerance, and WCS120 polypeptide accumulation in these genotypes proceeded at rates similar to those previously reported for 6 pC acclimation from 0 to 98 d. However, a differential accumulation of WCS120 polypeptide and expression of the COR genes Wcs120, Wcor410, and Wcor14 was observed in the leaf and crown tissues. COR gene transcript levels peaked at 2 d of the acclimation period in both tissues and differences among genotypes were most evident at this time. COR gene expression was highest for the LT-tolerant and lowest for the tender genotypes. However, expression rates were divergent enough in genotypes with intermediate hardiness that comparisons among tissues and/or times during acclimation often resulted in variable interpretations of the relative expression of the COR genes in the determination of LT tolerance. These observations emphasize the need to pay close attention to experimental conditions, sampling times, and genotype and tissue selection in experiments designed to identify the critical genetic components that interact to determine LT acclimation.
机译：小麦（Triticum aestivum L.）的低温（LT）耐受性获得中涉及许多COR基因（Cold调控基因）。这项研究比较了小麦近等基因系叶片和冠状组织中所选COR基因的相对表达模式，以加深对LT驯化的分子机制的了解。产生了相互接近的等基因系，从而在春季cv中互换了主要的Vrn-A1和隐性的vrn-A1基因座。曼尼通和冬季简历。北星这些基因型的物候学发展，LT耐受性获得和WCS120多肽积累的速率与先前报道的0到98 d适应6 pC的速率相似。但是，在叶和冠状组织中观察到WCS120多肽的差异积累和COR基因Wcs120，Wcor410和Wcor14的表达。在两个组织中，COR基因的转录水平均在适应期的第2天达到峰值，而此时基因型之间的差异最为明显。 COR基因的表达对LT耐受最高，而对于嫩基因型最低。但是，在具有中等耐性的基因型中，表达率差异足够大，以至于在适应过程中组织和/或时间之间的比较通常会导致在确定LT耐受性时对COR基因的相对表达产生不同的解释。这些观察结果强调了在设计实验中需要密切注意实验条件，采样时间，基因型和组织选择，以识别相互作用的关键遗传成分来确定LT适应性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号