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首页> 外文期刊>Comparative and functional genomics >Global Transcriptional Analysis of Olfactory Genes in the Head of Pine Shoot Beetle,Tomicus yunnanensis
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Global Transcriptional Analysis of Olfactory Genes in the Head of Pine Shoot Beetle,Tomicus yunnanensis

机译：云南Tomicus松树甲虫头部嗅觉基因的全球转录分析

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摘要

The most important proteins involved in olfaction include odorant binding protein (OBP), chemosensory protein (CSP), olfactory receptor (OR), and gustatory receptor (GR). Despite that the exhaustive genomic analysis has revealed a large number of olfactory genes in a number of model insects, it is still poorly understood for most nonmodel species. This is mostly due to the reason that the small antenna is challenging for collection. We can generally isolate one or few genes at a time by means of the traditional method. Here, we present the large-scale identifying members of the main olfactory genes from the head ofTomicus yunnanensisusing Illumina sequencing. In a single run, we obtained over 51.8 million raw reads. These reads were assembled into 57,142 unigenes. Nearly 29,384 of them were functionally annotated in the NCBI nonredundant database. By depth analysis of the data, 11 OBPs, 8 CSPs, 18 ORs, and 8 GRs were retrieved. Sequences encoding full length proteins were further characterised for one OBP and two CSPs. The obtained olfactory genes provide a major resource in further unraveling the molecular mechanisms ofT. yunnanensischemoperception. This study indicates that the next generation sequencing is an attractive approach for efficient identification of olfactory genes from insects, for which the genome sequence is unavailable.
机译：嗅觉中涉及的最重要的蛋白质包括气味结合蛋白（OBP），化学感应蛋白（CSP），嗅觉受体（OR）和味觉受体（GR）。尽管详尽的基因组分析已在许多模型昆虫中发现了大量嗅觉基因，但对于大多数非模型物种仍知之甚少。这主要是由于小天线难以收集的原因。我们通常可以通过传统方法一次分离一个或几个基因。在这里，我们介绍了使用Illumina测序从云南Tomicus头中的主要嗅觉基因的大规模鉴定成员。单次运行，我们获得了超过5180万次的原始读取。这些读段被组装成57142个单基因。在NCBI非冗余数据库中，将近29,384个在功能上进行了注释。通过对数据的深度分析，检索了11个OBP，8个CSP，18个OR和8个GR。对于一个OBP和两个CSP，进一步表征了编码全长蛋白质的序列。获得的嗅觉基因为进一步揭示T的分子机制提供了主要资源。云南化学感受。这项研究表明，下一代测序是一种有效鉴定昆虫嗅觉基因的诱人方法，而该基因组序列尚不可用。

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