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首页> 外文期刊>Comparative and functional genomics >In SilicoIdentification and Comparative Genomics of Candidate Genes Involved in Biosynthesis and Accumulation of Seed Oil in Plants
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In SilicoIdentification and Comparative Genomics of Candidate Genes Involved in Biosynthesis and Accumulation of Seed Oil in Plants

机译：植物种子油的生物合成和积累的候选基因的计算机鉴定和比较基因组学

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摘要

Genes involved in fatty acids biosynthesis, modification and oil body formation are expected to be conserved in structure and function in different plant species. However, significant differences in the composition of fatty acids and total oil contents in seeds have been observed in different plant species. Comparative genomics was performed on 261 genes involved in fatty acids biosynthesis, TAG synthesis, and oil bodies formation in Arabidopsis,Brassica rapa, castor bean and soybean.In silicoexpression analysis revealed that stearoyl desaturase, FatB, FAD2, oleosin and DGAT are highly abundant in seeds, thereby considered as ideal candidates for mining of favorable alleles in natural population. Gene structure analysis for major genes, ACCase, FatA, FatB, FAD2, FAD3 and DGAT, which are known to play crucial role in oil synthesis revealed that there are uncommon variations (SNPs and INDELs) which lead to varying content and composition of fatty acids in seed oil. The predicted variations can provide good targets for seed oil QTL identification, understanding the molecular mechanism of seed oil accumulation, and genetic modification to enhance seed oil yield in plants.
机译：预期脂肪酸生物合成，修饰和油体形成中涉及的基因在不同植物物种中在结构和功能上都是保守的。然而，在不同的植物物种中，观察到种子中脂肪酸组成和总油含量的显着差异。对拟南芥，芸苔，蓖麻子和大豆中脂肪酸生物合成，TAG合成和油体形成中涉及的261个基因进行了比较基因组学研究。硅表达分析显示，硬脂酰去饱和酶，FatB，FAD2，油质蛋白和DGAT含量很高。种子，因此被认为是在自然种群中挖掘有利等位基因的理想候选者。对主要基因ACCase，FatA，FatB，FAD2，FAD3和DGAT的基因结构分析在油合成中起着至关重要的作用，发现存在罕见的变异（SNP和INDEL），导致脂肪酸含量和组成的变化在种子油中。预测的变异可以为种子油QTL鉴定，了解种子油积累的分子机制以及提高植物种子油产量的基因修饰提供良好的目标。

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