Evaluation of Sensitivity and Specificity of a Mycoplasma haemomuris-Specific Polymerase Chain Reaction Test

Zhang; Chunbin; nbsp; Rikihisa; Yasuko

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Evaluation of Sensitivity and Specificity of a Mycoplasma haemomuris-Specific Polymerase Chain Reaction Test

机译：血统支原体特异性聚合酶链反应试验的敏感性和特异性评估

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BackgroundandPurpose:Mycoplasmahaemomuris,asmallpleomorphicbacteriumparasiticofredbloodcells,oftencauseschronicandsubclinicalinfectionofrodents.Mycoplasmahaemomurisisuncultivable,andaserologictestingmethodisnotreadilyavailable.Thepurposeofthestudyreportedherewastodevelopasensitiveandspecificpolymerasechainreaction(PCR)testfordetectionofM.haemomurisinbloodsamples.brxmlns="http://pub2web.metastore.ingenta.coms/"/brMethods:OnthebasisoftheregionsoftheM.haemomuris16SrRNAgenemostdivergentfromcorrespondingregionsofrelatedbacteria,M.haemomuris-specificprimersweredesignedsothattheseprimerscouldselectivelyamplifyM.haemomurisDNA.APCRtestwasperformed,usingbloodsamplesfromBALB/cmiceinfectedwithM.haemomurisstrainsTR8564,TR8563,andTR8556.brxmlns="http://pub2web.metastore.ingenta.coms/"/brResults:UseofthePCRtestenableddetectionofM.haemomurisDNAinaminimumof0.0001#59157;lofinfectedmouseblood.ThetestalsowasspecificforM.haemomurisanddidnotamplifycloselyrelatedspecies,suchasM.haemofelis,M.haemosuis,M.orale,orAnaplasmamarginale.brxmlns="http://pub2web.metastore.ingenta.coms/"/brConclusion:ThismethodissensitiveandspecificfordetectionofM.haemomuris.

机译：BackgroundandPurpose：Mycoplasmahaemomuris，asmallpleomorphicbacteriumparasiticofredbloodcells，oftencauseschronicandsubclinicalinfectionofrodents.Mycoplasmahaemomurisisuncultivable，andaserologictestingmethodisnotreadilyavailable.Thepurposeofthestudyreportedherewastodevelopasensitiveandspecificpolymerasechainreaction（PCR）testfordetectionofM.haemomurisinbloodsamples 方法：OnthebasisoftheregionsoftheM.haemomuris16SrRNAgenemostdivergentfromcorrespondingregionsofrelatedbacteria，设计了M.haemomuris特异的引物，以便这些引物可以选择性扩增M.haemomuris DNA.APCR测试是通过使用感染了M.haemomuriss菌株TR8564，TR8563和TR8556的BALB / cmice中的血样进行的。 0001＃59157;该病还存在最终感染的小鼠血液。该测试还针对血红霉菌和双歧杆菌，放大了与之相关的物种，例如血红霉菌，血红霉菌suis，M.orale或Anaplasmamarginale。结论：这种方法对检测血红霉菌敏感。

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《Comparative Medicine》 |2002年第4期|共页
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Zhang; Chunbin; nbsp; Rikihisa; Yasuko;
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中图分类畜牧、动物医学、狩猎、蚕、蜂;
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Evaluation of Sensitivity and Specificity of a Mycoplasma haemomuris-Specific Polymerase Chain Reaction Test

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