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首页> 外文期刊>Biology Open >Human trophoblasts are primarily distinguished from somatic cells by differences in the pattern rather than the degree of global CpG methylation
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Human trophoblasts are primarily distinguished from somatic cells by differences in the pattern rather than the degree of global CpG methylation

机译：人类滋养细胞的区别主要在于体细胞与体细胞的区别，而不是总体CpG甲基化程度

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The placenta is a fetal exchange organ connecting mother and baby that facilitates fetal growthin utero. DNA methylation is thought to impact placental development and function. Global DNA methylation studies using human placental lysates suggest that the placenta is uniquely hypomethylated compared to somatic tissue lysates, and this hypomethylation is thought to be important in conserving the unique placental gene expression patterns required for successful function. In the placental field, methylation has frequently been examined in tissue lysates, which contain mixed cell types that can confound results. To better understand how DNA methylation influences placentation, DNA from isolated first trimester trophoblast populations underwent reduced representation bisulfite sequencing and was compared to publicly available data of blastocyst-derived and somatic cell populations. First, this revealed that, unlike murine blastocysts, human trophectoderm and inner cell mass samples did not have significantly different levels of global methylation. Second, our work suggests that differences in global CpG methylation between trophoblasts and somatic cells are much smaller than previously reported. Rather, our findings suggest that different patterns of CpG methylation may be more important in epigenetically distinguishing the placenta from somatic cell populations, and these patterns of methylation may contribute to successful placental/trophoblast function.
机译：胎盘是连接母亲和婴儿的胎儿交换器官，可促进子宫内胎儿的生长。 DNA甲基化被认为会影响胎盘的发育和功能。使用人胎盘裂解物进行的全球DNA甲基化研究表明，与体细胞组织裂解物相比，胎盘具有唯一的低甲基化，这种低甲基化被认为对于保存成功功能所需的独特胎盘基因表达模式非常重要。在胎盘领域，经常在组织裂解物中检查甲基化，该裂解物中含有可能混淆结果的混合细胞类型。为了更好地理解DNA甲基化如何影响胎盘形成，从分离的早孕滋养层细胞群体中提取的DNA经历了代表性的亚硫酸氢盐测序，并与囊胚来源和体细胞群体的公开数据进行了比较。首先，这表明，与鼠胚泡不同，人类滋养外胚层和内部细胞团样本的总体甲基化水平没有显着差异。其次，我们的工作表明，滋养层细胞与体细胞之间总体CpG甲基化的差异比以前报道的要小得多。而是，我们的发现表明，不同的CpG甲基化模式可能在表观遗传上将胎盘与体细胞群区分开来更为重要，而这些甲基化模式可能有助于成功的胎盘/滋养细胞功能。

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