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Detecting DNA methylation patterns in genomic DNA using bisulfite-catalyzed transamination of CpGS
Detecting DNA methylation patterns in genomic DNA using bisulfite-catalyzed transamination of CpGS
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机译:使用亚硫酸氢盐催化的CpGS转氨作用检测基因组DNA中的DNA甲基化模式
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摘要
Methods and compositions for identifying or detecting methylation patterns in a target nucleic acid are described. Cytosine residues in regions of interest are transaminated and labeled. The labeled residues are then hybridized to a microarray containing probes complementary to the region of interest, to identify the amount of methylation in the target nucleic acid.
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