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首页> 外文期刊>Biology Open >Hepatic glucose metabolic responses to digestible dietary carbohydrates in two isogenic lines of rainbow trout
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Hepatic glucose metabolic responses to digestible dietary carbohydrates in two isogenic lines of rainbow trout

机译：虹鳟鱼两个等基因系对可消化食物碳水化合物的肝葡萄糖代谢反应

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Rainbow trout (Oncorhynchus mykiss) was recognized as a typical ‘glucose-intolerant’ fish and poor dietary carbohydrate user. Our first objective was to test the effect of dietary carbohydrates themselves (without modification of dietary protein intake) on hepatic glucose gene expression (taking into account the paralogs). The second aim was to research if two isogenic trout lines had different responses to carbohydrate intake, showing one with a better use dietary carbohydrates. Thus, we used two isogenic lines of rainbow trout (named A32h and AB1h) fed with either a high carbohydrate diet or a low carbohydrate diet for 12?weeks. We analysed the zootechnical parameters, the plasma metabolites, the hepatic glucose metabolism at the molecular level and the hormonal-nutrient sensing pathway. Globally, dietary carbohydrate intake was associated with hyperglycaemia and down regulation of the energy sensor Ampk, but also with atypical regulation of glycolysis and gluconeogenesis in the liver. Indeed, the first steps of glycolysis and gluconeogenesis catalysed by the glucokinase and the phospenolpyruvate carboxykinase are regulated at the molecular level by dietary carbohydrates as expected (i.e. induction of the glycolyticgckand repression of the gluconeogenicpck); by contrast, and surprisingly, for two other key glycolytic enzymes (phosphofructokinase enzyme –pfkland pyruvate kinase –pk) some of the paralogs (pfklbandpklr) are inhibited by carbohydrates whereas some of the genes coding gluconeogenic enzymes (the glucose-6-phosphatase enzymeg6pcb1bandg6pcb2agene and the fructose1-6 biphosphatase paralogfbp1a) are induced. On the other hand, some differences for the zootechnical parameters and metabolic genes were also found between the two isogenic lines, confirming the existence of genetic polymorphisms for nutritional regulation of intermediary metabolism in rainbow trout. In conclusion, our study determines some new and unexpected molecular regulations of the glucose metabolism in rainbow trout which may partly lead to the poor utilization of dietary carbohydrates and it underlines the existence of differences in molecular regulation of glucose metabolism between two isogenic lines which provides arguments for future selection of rainbow trout.
机译：虹鳟鱼（Oncorhynchus mykiss）被认为是典型的“不耐葡萄糖”的鱼类，且饮食中碳水化合物的摄入较差。我们的首要目标是测试饮食碳水化合物本身（不改变饮食蛋白质摄入量）对肝葡萄糖基因表达的影响（考虑旁系同源物）。第二个目的是研究两个同基因鳟鱼品系对碳水化合物的摄入是否有不同的反应，表明其中一个具有更好的饮食碳水化合物。因此，我们使用了两个虹鳟鱼的同基因系（分别命名为A32h和AB1h），分别饲喂高碳水化合物饮食或低碳水化合物饮食12周。我们分析了动物技术参数，血浆代谢物，肝糖代谢的分子水平和激素-营养素的传感途径。在全球范围内，饮食中碳水化合物的摄入与高血糖症和能量传感器Ampk的下调有关，但也与肝脏中糖酵解和糖异生的非典型调节有关。实际上，由葡萄糖激酶和磷酸丙酮丙酮酸羧激酶催化的糖酵解和糖异生的第一步如预期的那样在饮食水平上由饮食碳水化合物调节（即诱导糖酵解和抑制糖原异生性）。相比之下，令人惊讶的是，对于其他两种关键的糖酵解酶（磷酸果糖激酶酶–pfkland丙酮酸激酶–pk），某些旁系同源物（pfklbandpklr）被碳水化合物抑制，而某些编码糖异生酶的基因（葡萄糖6磷酸酶g6pcb1bandg6pcb2agene和果糖1-6双磷酸酶paralogfbp1a）被诱导。另一方面，在两个等基因系之间还发现了动物技术参数和代谢基因的一些差异，这证实了虹鳟中间代谢营养调节的遗传多态性的存在。总而言之，我们的研究确定了虹鳟鱼葡萄糖代谢的一些新的和出乎意料的分子调控，这可能部分导致膳食碳水化合物利用不足，它突显了两个等基因系之间葡萄糖代谢的分子调控存在差异，这提供了论点供将来选择虹鳟鱼。

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