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首页> 外文期刊>Clinical & developmental immunology. >A Possible Role of HMGB1 in DNA Demethylation in CD4 + T Cells from Patients with Systemic Lupus Erythematosus
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A Possible Role of HMGB1 in DNA Demethylation in CD4 + T Cells from Patients with Systemic Lupus Erythematosus

机译：HMGB1在系统性红斑狼疮患者CD4 + T细胞DNA脱甲基中的可能作用

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The aberrant activity of CD4~(+)T cells in patients with systemic lupus erythematosus (SLE) is associated with DNA hypomethylation of the regulatory regions in CD11a and CD70 genes. Our previous studies demonstrated that Gadd45a contributes to the development of SLE by promoting DNA demethylation in CD4~(+)T cells. In this study, we identified proteins that bind to Gadd45a in CD4~(+)T cells during SLE flare by using the method of co-immunoprecipitation and mass spectrometry, High mobility group box protein 1 (HMGB1) is one of identified proteins. Furthermore, gene and protein expression of HMGB1 was significantly increased in SLE CD4~(+)T cells compared to controls, and HMGB1 mRNA was correlated with CD11a and CD70 mRNA. A significant, positive correlation was found between HMGB1 mRNA and SLEDAI for SLE patients. Our data demonstrate that HMGB1 binds to Gadd45a and may be involved in DNA demethylation in CD4~(+)T cells during lupus flare.
机译：系统性红斑狼疮（SLE）患者CD4〜（+）T细胞的异常活性与CD11a和CD70基因调控区的DNA甲基化不足有关。我们以前的研究表明，Gadd45a通过促进CD4〜（+）T细胞中的DNA脱甲基作用而有助于SLE的发展。在这项研究中，我们使用共免疫沉淀和质谱法鉴定了SLE耀斑期间CD4〜（+）T细胞中与Gadd45a结合的蛋白，高迁移率基盒蛋白1（HMGB1）是鉴定出的蛋白之一。此外，与对照相比，SLE CD4〜（+）T细胞中HMGB1的基因和蛋白质表达显着增加，并且HMGB1 mRNA与CD11a和CD70 mRNA相关。发现SLE患者HMGB1 mRNA与SLEDAI之间存在显着的正相关。我们的数据表明，HMGB1与Gadd45a结合，可能在狼疮发作期间参与CD4〜（+）T细胞的DNA脱甲基化。

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