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首页> 外文期刊>Biology Open >Innovative approaches to establish and characterize primary cultures: an?ex vivo?3D system and the zebrafish model
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Innovative approaches to establish and characterize primary cultures: an?ex vivo?3D system and the zebrafish model

机译：建立和表征原始文化的创新方法：“体外” 3D系统和斑马鱼模型

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Patient-derived specimens are an invaluable resource to investigate tumor biology. However,?in vivo?studies on primary cultures are often limited by the small amount of material available, while conventional?in vitro?systems might alter the features and behavior that characterize cancer cells. We present our data obtained on primary dedifferentiated liposarcoma cells cultured in a 3D scaffold-based system and injected into a zebrafish model. Primary cells were characterized?in vitro?for their morphological features, sensitivity to drugs and biomarker expression, and?in vivo?for their engraftment and invasiveness abilities. The 3D culture showed a higher enrichment in cancer cells than the standard monolayer culture and a better preservation of liposarcoma-associated markers. We also successfully grafted primary cells into zebrafish, showing their local migratory and invasive abilities. Our work provides proof of concept of the ability of 3D cultures to maintain the original phenotype of?ex vivo?cells, and highlights the potential of the zebrafish model to provide a versatile?in vivo?system for studies with limited biological material. Such models could be used in translational research studies for biomolecular analyses, drug screenings and tumor aggressiveness assays.
机译：患者来源的标本是研究肿瘤生物学的宝贵资源。然而，原代培养的体内研究通常受到少量可用材料的限制，而常规的体外系统可能会改变表征癌细胞的特征和行为。我们介绍我们在基于3D支架的系统中培养并注入斑马鱼模型中的原代去分化脂肪肉瘤细胞获得的数据。原代细胞的体外形态，对药物和生物标志物的敏感性以及在体内的植入和侵袭能力被表征。 3D培养物比标准的单层培养物显示出更高的癌细胞富集度，并更好地保存了与脂肪肉瘤相关的标志物。我们还成功地将原代细胞移植到了斑马鱼中，展示了它们的局部迁移和侵袭能力。我们的工作为3D培养物维持“离体”细胞原始表型的能力提供了概念证明，并强调了斑马鱼模型为有限的生物材料研究提供通用的“活体内”系统的潜力。这样的模型可以用于生物分子分析，药物筛选和肿瘤侵袭性测定的转化研究中。

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