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首页> 外文期刊>Biology Open >Analysis of novel hyperosmotic shock response suggests ‘beads in liquid’ cytosol structure
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Analysis of novel hyperosmotic shock response suggests ‘beads in liquid’ cytosol structure

机译：对新型高渗休克反应的分析表明“液体中的珠粒”细胞溶胶结构

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Proteins can aggregate in response to stresses, including hyperosmotic shock. Formation and disassembly of aggregates is a relatively slow process. We describe a novel instant response of the cell to hyperosmosis, during which chaperones and other proteins form numerous foci with properties uncharacteristic of classical aggregates. These foci appeared/disappeared seconds after shock onset/removal, in close correlation with cell volume changes. Genome-wide and targeted testing revealed chaperones, metabolic enzymes, P-body components and amyloidogenic proteins in the foci. Most of these proteins can form large assemblies and for some, the assembled state was pre-requisite for participation in foci. A genome-wide screen failed to identify genes whose absence prevented foci participation by Hsp70. Shapes of and interconnections between foci, revealed by super-resolution microscopy, indicated that the foci were compressed between other entities. Based on our findings, we suggest a new model of cytosol architecture as a collection of numerous gel-like regions suspended in a liquid network. This network is reduced in volume in response to hyperosmosis and forms small pockets between the gel-like regions.
机译：蛋白质可对压力（包括高渗性休克）作出反应而聚集。聚集体的形成和分解是一个相对缓慢的过程。我们描述了细胞对高渗的新型即时反应，在此期间伴侣和其他蛋白质形成许多病灶，具有经典聚集体不具有的特性。这些病灶在电击发作/去除后几秒钟出现/消失，与细胞体积变化密切相关。全基因组和有针对性的测试揭示了病灶中的伴侣，代谢酶，P体成分和淀粉样蛋白。这些蛋白质中的大多数可以形成大型装配体，对于某些装配体而言，装配状态是参与病灶的前提条件。全基因组筛选无法识别其缺失阻止了Hsp70参与灶的基因。超分辨显微镜显示的病灶形状和病灶之间的相互联系表明，病灶在其他实体之间受压。根据我们的发现，我们提出了一种新的细胞溶胶结构模型，该模型是悬浮在液体网络中的许多凝胶状区域的集合。响应高渗，该网络的体积减小，并在凝胶状区域之间形成小袋。

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