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首页> 外文期刊>Biology Open >The 17β-estradiol induced upregulation of the adhesion G-protein coupled receptor (ADGRG7) is modulated by ESRα and SP1 complex
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The 17β-estradiol induced upregulation of the adhesion G-protein coupled receptor (ADGRG7) is modulated by ESRα and SP1 complex

机译：ESRα和SP1复合物可调节17β-雌二醇诱导的粘附G蛋白偶联受体（ADGRG7）的上调

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The physiological role and the regulation of ADGRG7 are not yet elucidated. The functional involvement of this receptor was linked with different physiological process such as reduced body weight, gastrointestinal function and recently, a gene variant in ADGRG7 was observed in patients with adolescent idiopathic scoliosis. Here, we identify theADGRG7as an estrogen-responsive gene under the regulation of estrogen receptor ERα in scoliotic osteoblasts and other cells lines. We found thatADGRG7expression was upregulated in response to estrogen (E2) in adolescent idiopathic scoliosis (AIS) cells.ADGRG7promoter studies indicate the presence of an ERα response half site in close vicinity of a specificity protein 1 (SP1) binding site. Mutation of the SP1 site completely abrogated the response to E2, indicating its essential requirement. ChIP confirmed the binding of SP1 and ERα to theADGRG7promoter. Our results identify theADGRG7gene as an estrogen-responsive gene under the control of ERα and SP1 tethered actions, suggesting a possible role of estrogens in the regulation ofADGRG7.This article has an associated First Person interview with the first author of the paper.
机译：尚未阐明ADGRG7的生理作用和调节。该受体的功能参与与不同的生理过程有关，例如体重减轻，胃肠道功能下降，最近，在青少年特发性脊柱侧弯患者中发现了ADGRG7的基因变异。在这里，我们确定在脊柱侧凸成骨细胞和其他细胞系中，在雌激素受体ERα的调节下，ADGRG7是雌激素反应性基因。我们发现在青少年特发性脊柱侧凸（AIS）细胞中，ADGRG7的表达上调是对雌激素（E2）的响应.ADGRG7启动子研究表明，ERα响应半位点存在于特异性蛋白1（SP1）结合位点附近。 SP1位点的突变完全废除了对E2的响应，表明其基本要求。 ChIP确认SP1和ERα与ADGRG7启动子结合。我们的结果确定了ADGRG7基因是受ERα和SP1束缚作用控制的雌激素响应基因，表明雌激素可能在ADGRG7的调控中发挥作用。本文与第一人相关的第一人称访谈。

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