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首页> 外文期刊>Clinical and diagnostic laboratory immunology >In vitro immunologic and virologic effects of interleukin 15 on peripheral blood mononuclear cells from normal donors and human immunodeficiency virus type 1-infected patients.
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In vitro immunologic and virologic effects of interleukin 15 on peripheral blood mononuclear cells from normal donors and human immunodeficiency virus type 1-infected patients.

机译：白细胞介素15对正常供体和1型人类免疫缺陷病毒感染患者外周血单个核细胞的体外免疫和病毒学作用。

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Interleukin 15 (IL-15) is a cytokine that shares receptor subunits and functional activity, such as T-cell and B-cell stimulation, with IL-2. The effect of IL-2 on immune function and human immunodeficiency virus (HIV) viral load in HIV-infected patients is being actively studied. Thus, we examined how IL-15 compares with IL-2 in several in vitro immunologic and virologic assays in order to explore whether a rationale exists for pursuing initial clinical therapeutic trials with IL-15. The effects of IL-15 on induction of lymphokine-activated killer (LAK) cells, gamma interferon (IFN-gamma) production from HIV-positive peripheral blood mononuclear cells (PBMCs), and HIV production from PBMCs were studied. Induction of LAK cells by IL-15 was found in eight of eight HIV-positive donors. Incubation of PBMCs from some donors with IL-15 (1, 10, 50, and 100 ng/ml) induced production of IFN-gamma. The effect of IL-15 was compared with that of IL-2 on HIV replication in PBMCs from five HIV-positive patients and four HIV-negative donors whose PBMCs were infected in vitro with HIV. Levels of HIV p24 antigen were moderately lower in the presence of 10 ng of IL-15 per ml than with 10 ng of IL-2 per ml, but they were similar for 100 and 500 ng of each cytokine per ml. In summary, IL-15 can induce LAK cell activity in HIV-seropositive patients and can stimulate IFN-gamma production from PBMCs of some donors. IL-15 stimulates levels of HIV production from PBMCs which are similar to or moderately lower than those obtained with IL-2, depending on cytokine concentration.
机译：白介素15（IL-15）是一种与IL-2共享受体亚基和功能活性（例如T细胞和B细胞刺激）的细胞因子。正在积极研究IL-2对HIV感染患者的免疫功能和人类免疫缺陷病毒（HIV）病毒载量的影响。因此，我们探讨了几种体外免疫和病毒学检测方法中IL-15与IL-2的比较，以探讨是否存在进行IL-15初始临床治疗试验的理由。研究了IL-15对诱导淋巴因子激活的杀伤（LAK）细胞，从HIV阳性外周血单核细胞（PBMC）产生γ-干扰素（IFN-γ）和从PBMC产生HIV的影响。在八个HIV阳性供体中的八个中发现了IL-15对LAK细胞的诱导作用。将来自某些供体的PBMC与IL-15（1、10、50和100 ng / ml）一起孵育可诱导产生IFN-γ。比较了IL-15和IL-2对5例HIV阳性患者和4例PBMC在体外被HIV感染的HIV阴性供体的PBMC中HIV复制的影响。在每毫升10 ng IL-15的情况下，HIV p24抗原的水平要比在每毫升10 ng IL-2的情况下适度降低，但是对于每毫升100和500 ng的每种细胞因子，它们的水平相似。总之，IL-15可以诱导HIV血清阳性患者的LAK细胞活性，并可以刺激某些供体的PBMC产生IFN-γ。 IL-15刺激PBMC产生HIV的水平与依IL-2所获得的水平相近或略低，具体取决于细胞因子的浓度。

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