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首页> 外文期刊>Transfusion: The Journal of the American Association of Blood Banks >Absence of detectable xenotropic murine leukemia virus-related virus in plasma or peripheral blood mononuclear cells of human immunodeficiency virus Type 1-infected blood donors or individuals in Africa.
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Absence of detectable xenotropic murine leukemia virus-related virus in plasma or peripheral blood mononuclear cells of human immunodeficiency virus Type 1-infected blood donors or individuals in Africa.

机译：在非洲，人类免疫缺陷病毒1型感染的献血者或个人的血浆或外周血单核细胞中没有可检测的异源鼠白血病病毒相关病毒。

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摘要

BACKGROUND: Since the identification of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer patients in 2006 and in chronic fatigue syndrome patients in 2009, conflicting findings have been reported regarding its etiologic role in human diseases and prevalence in general populations. In this study, we screened both plasma and peripheral blood mononuclear cells (PBMNCs) collected in Africa from blood donors and human immunodeficiency virus Type 1 (HIV-1)-infected individuals to gain evidence of XMRV infection in this geographic region. STUDY DESIGN AND METHODS: A total of 199 plasma samples, 19 PBMNC samples, and 50 culture supernatants from PBMNCs of blood donors from Cameroon found to be infected with HIV-1 and HIV-1 patients from Uganda were screened for XMRV infection using a sensitive nested polymerase chain reaction (PCR) or reverse transcription (RT)-PCR assay. RESULTS: Using highly sensitive nested PCR or RT-PCR and real-time PCR assays capable of detecting at least 10 copies of XMRV plasmid DNA per reaction, none of the 268 samples tested were found to be XMRV DNA or RNA positive. CONCLUSIONS: Our results failed to demonstrate the presence of XMRV infection in African blood donors or individuals infected with HIV-1. More studies are needed to understand the prevalence, epidemiology, and geographic distribution of XMRV infection worldwide.
机译：摘要背景：自从2006年在前列腺癌患者和2009年在慢性疲劳综合征患者中发现异种鼠白血病病毒相关病毒（XMRV）以来，关于其在人类疾病中的病因学作用和普通人群中的患病率的报道一直存在矛盾的发现。在这项研究中，我们筛选了在非洲从献血者和人类免疫缺陷病毒1型（HIV-1）感染者中收集的血浆和外周血单个核细胞（PBMNC），以获取该地区XMRV感染的证据。研究设计与方法：使用敏感试剂筛选了喀麦隆献血者被发现感染了HIV-1和HIV-1的喀麦隆献血者的199份血浆样本，19份PBMNC样本和50份培养上清液，以进行XMRV感染筛查。巢式聚合酶链反应（PCR）或逆转录（RT）-PCR分析。结果：使用能够检测每个反应至少10份XMRV质粒DNA的高度敏感的巢式PCR或RT-PCR和实时PCR分析，发现268个样本中没有发现XMRV DNA或RNA阳性。结论：我们的结果未能证明非洲献血者或感染HIV-1的个体存在XMRV感染。需要更多的研究来了解全球XMRV感染的流行，流行病学和地理分布。

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