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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Immunoblot analysis for serodiagnosis of tuberculosis using a 45/47-kilodalton antigen complex of Mycobacterium tuberculosis.
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Immunoblot analysis for serodiagnosis of tuberculosis using a 45/47-kilodalton antigen complex of Mycobacterium tuberculosis.

机译：使用结核分枝杆菌的45 / 47-千达尔顿抗原复合物对结核进行血清学诊断的免疫印迹分析。

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摘要

We evaluated the immunoglobulin G (IgG) antibody response to the 45/47-kDa secreted protein of Mycobacterium tuberculosis by immunoblot assay, to assess its potential value for serological diagnosis. Control subjects consisted of healthy volunteers with negative or positive tuberculin skin tests. Most (>98%) scored negative in an immunoblot test when the sera were analyzed at a 1:400 dilution. Approximately 40% of sera (diluted 1 in 400) from tuberculous patients (positive smears) recognized the antigen complex. The sensitivity of the test for patients suffering from extrapulmonary tuberculosis was similar to that for patients suffering from pulmonary tuberculosis but who had negative smears. The frequency of positive reactions among the patients suffering from other pulmonary diseases was similar to that among the control subjects. In tuberculous patients infected with human immunodeficiency virus, the sensitivity of the immunoblot test was significantly lower. Thus, this test based on an antigen complex used in an immunoblot assay to detect the presence of IgG antibody has a specificity of 98% and a sensitivity of 40%. The simultaneous use of different purified antigens, selected at the same high specificity level, may improve the sensitivity of such an assay.
机译：我们通过免疫印迹法评估了对结核分枝杆菌45 / 47-kDa分泌蛋白的免疫球蛋白G（IgG）抗体反应，以评估其在血清学诊断中的潜在价值。对照对象包括结核菌素皮肤试验阴性或阳性的健康志愿者。当以1：400稀释度分析血清时，大多数（> 98％）分数在免疫印迹测试中均为阴性。结核病患者（阳性涂片）约40％的血清（400份稀释1次）识别抗原复合物。该测试对肺外结核患者的敏感性与对肺结核但涂片阴性的患者的敏感性相似。在患有其他肺部疾病的患者中，阳性反应的频率与对照组的相似。在感染了人类免疫缺陷病毒的结核病患者中，免疫印迹测试的敏感性明显降低。因此，该基于用于免疫印迹测定中以检测IgG抗体的存在的抗原复合物的测试具有98％的特异性和40％的灵敏度。同时使用以相同高特异性水平选择的不同纯化抗原可以提高此类测定的灵敏度。

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