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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Multisite Comparison of CD4 and CD8 T-Lymphocyte Counting by Single- versus Multiple-Platform Methodologies: Evaluation of Beckman Coulter Flow-Count Fluorospheres and the tetraONE System
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Multisite Comparison of CD4 and CD8 T-Lymphocyte Counting by Single- versus Multiple-Platform Methodologies: Evaluation of Beckman Coulter Flow-Count Fluorospheres and the tetraONE System

机译：通过单平台方法和多平台方法对CD4和CD8 T淋巴细胞计数的多点比较：Beckman Coulter流量计数荧光球和tetraONE系统的评估

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New analytic methods that permit absolute CD4 and CD8 T-cell determinations to be performed entirely on the flow cytometer have the potential for improving assay precision and accuracy. In a multisite trial, we compared two different single-platform assay methods with a predicate two-color assay in which the absolute lymphocyte count was derived by conventional hematology. A two-color method employing lymphocyte light scatter gating and Beckman Coulter Flow-Count fluorospheres for absolute counting produced within-laboratory precision equivalent to that of the two-color predicate method, as measured by coefficient of variation of replicate measurements. The fully automated Beckman Coulter tetraONE System four-color assay employing CD45 lymphocyte gating, automated analysis, and absolute counting by fluorospheres resulted in a small but significant improvement in the within-laboratory precision of CD4 and CD8 cell counts and percentages suggesting that the CD45 lymphocyte gating and automated analysis might have contributed to the improved performance. Both the two-color method employing Flow-Count fluorospheres and the four-color tetraONE System provided significant and substantial improvements in between-laboratory precision of absolute counts. In some laboratories, absolute counts obtained by the single-platform methods showed small but consistent differences relative to the predicate method. Comparison of each laboratory's absolute counts with the five-laboratory median value suggested that these differences resulted from a bias in the absolute lymphocyte count obtained from the hematology instrument in some laboratories. These results demonstrate the potential for single-platform assay methods to improve within-laboratory and between-laboratory precision of CD4 and CD8 T-cell determinations compared with conventional assay methods.
机译：允许完全在流式细胞仪上进行绝对CD4和CD8 T细胞测定的新分析方法具有提高测定精度和准确性的潜力。在一项多站点试验中，我们将两种不同的单平台检测方法与谓词双色检测方法进行了比较，在该方法中，绝对淋巴细胞计数是通过常规血液学得出的。一种采用淋巴细胞光散射门控和Beckman Coulter Flow-Count荧光球进行绝对计数的双色方法，在实验室内的精确度与双色谓词方法相当，通过重复测量的变异系数进行测量。全自动Beckman Coulter tetraONE System四色测定法采用CD45淋巴细胞门控，自动分析和荧光球绝对计数，导致实验室内CD4和CD8细胞计数的精确度有了微小但显着的提高，其百分比表明CD45淋巴细胞门控和自动分析可能有助于提高性能。使用Flow-Count荧光球的双色方法和四色tetraONE系统都在绝对计数的实验室间精度上提供了显着和显着的提高。在某些实验室中，通过单平台方法获得的绝对计数显示出相对于谓词方法的微小但一致的差异。将每个实验室的绝对计数与五个实验室的中位数进行比较表明，这些差异是由于某些实验室从血液学仪器获得的绝对淋巴细胞计数存在偏差所致。这些结果证明，与传统的测定方法相比，单平台测定方法具有提高CD4和CD8 T细胞测定的实验室内和实验室间精度的潜力。

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