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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Susceptibility of Vibrio cholerae O139 to Antibody-Dependent, Complement-Mediated Bacteriolysis
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Susceptibility of Vibrio cholerae O139 to Antibody-Dependent, Complement-Mediated Bacteriolysis

机译：O139霍乱弧菌对抗体依赖性补体介导的细菌溶解的敏感性

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Volunteer studies with Vibrio cholerae O1 have shown that the best correlate of a vaccine's protective efficacy is its propensity to elicit serum bactericidal responses in its recipients. Attempts to detect such responses following infection with V. cholerae O139, however, have met with varying success. Using a tube-based assay which involves viable counting, we now report that strains of serogroup O139 can appear to be sensitive or resistant to a fixed concentration of complement in the presence of antibody, depending on assay conditions. Susceptibility to lysis is critically dependent on the availability of complement, but with O139 indicator strains this is not simply determined by the concentration of serum added to the reaction mix. The nature of the assay diluent and the concentration of indicator bacteria can also dramatically affect bactericidal end points, whereas such variables have minimal significance with O1 indicator bacteria. Although some laboratories use unencapsulated mutant strains to seek evidence of seroconversion following exposure to V. cholerae O139, this is not necessary, and our findings question the significance of capsule expression as a determinant of complement sensitivity when antibody is present. The medium used for growth of the indicator strain and the particular strain used appeared to be unimportant. Each of seven O139 isolates tested was found to be lysed by antibody and complement in our standard assay system, which allowed the detection of significant serum bactericidal responses in 9 of 11 cases of O139 disease.
机译：对霍乱弧菌 O1的志愿者研究表明，疫苗保护功效的最佳关联是其在受者中引起血清杀菌反应的倾向。尝试检测感染 V后的此类反应。霍乱弧菌O139取得了不同的成功。现在使用基于管的测定法，其中涉及可行的计数，我们报道血清O139菌株在存在抗体的情况下似乎对固定浓度的补体敏感或具有抗性，具体取决于测定条件。裂解的敏感性主要取决于补体的可用性，但是对于O139指示菌株，这并不简单地通过添加到反应混合物中的血清浓度来确定。测定稀释剂的性质和指示剂细菌的浓度也可以极大地影响杀菌终点，而这些变量对O1指示剂细菌的影响最小。尽管一些实验室使用未封装的突变菌株来寻找暴露于 V后血清转化的证据。 O139，这不是必需的，我们的发现质疑当抗体存在时胶囊表达作为补体敏感性决定因素的意义。用于指示菌株生长的培养基和所使用的特定菌株似乎并不重要。在我们的标准测定系统中，发现被测试的七个O139分离株均被抗体和补体裂解，从而可以检测11例O139疾病中9例的重要血清杀菌反应。

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