Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays

Blanca Velázquez; Hugo Massaldi; Julio Battistoni; José A. Chabalgoity

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Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays

机译：重组链球菌溶血素-O的构建，表达及在免疫分析中的应用价值

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Commercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.

机译：用于评估抗链球菌溶血素-O抗体的市售免疫测定使用通过复杂过程获得的天然链球菌溶血素-O。我们以更高的产率和更简单的纯化方法制备了具有生物活性的重组链球菌溶血素-O。用该重组体开发的酶联免疫吸附试验与商业试验显示出良好的相关性，表明它可能适用于免疫试验。

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《Clinical and diagnostic laboratory immunology》 |2005年第5期|共2页
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Blanca Velázquez; Hugo Massaldi; Julio Battistoni; José A. Chabalgoity;
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6. Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays [O] . Blanca Velázquez, Hugo Massaldi, Julio Battistoni, 2005

机译：重组链球菌溶血素-O的构建表达及在免疫分析中的应用价值
7. Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays [O] . Velázquez, Blanca, Massaldi, Hugo, Battistoni, Julio, 2005

机译：重组链球菌溶血素-O的构建，表达及在免疫分析中的应用价值

Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays

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