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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis
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Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis

机译：西方血吸虫病诊断试剂盒的开发和评估

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We evaluated the performance of Western blot (WB) analysis using commercially available antigen strips and compared the results with those of indirect hemagglutination (IHA) and indirect immunofluorescence (IFAT) for the serodiagnosis of human schistosomiasis. The antigen preparation was a crude extract of Schistosoma mansoni. The WB profile characteristics of schistosomiasis were characterized by comparing the results for 58 serum samples from patients with parasitologically proven S. mansoni (n = 12) and S. haematobium (n = 46) infections and 37 individuals with probable cases of schistosomiasis but with only positive serology results. The specificity of WB analysis was assessed by testing 12 serum samples from healthy subjects, 67 serum samples from patients with other proven helminthic and protozoan infections, and 16 serum samples from patients with autoantibodies. Six immunodominant bands (65, 70, 80, 95, 110, and 120 kDa) were revealed with sera from patients with schistosomiasis. The presence of three or more bands in the range 65 to 120 kDa, with the exception of the 100-kDa band, was considered diagnostic for Schistosoma infection and had a specificity of 100% in our series. In patients with proven schistosomiasis, the sensitivity of WB analysis was 84.5%, whereas those of IFAT and IHA were 65.5 and 72.9%, respectively. For serologically proven cases, the sensitivity of WB analysis was 97.3%. The overall sensitivity and specificity for both groups of patients were 89.5 and 100%, respectively, with positive and negative predictive values of 100 and 91.3%, respectively. We conclude that WB analysis is a useful technique for the immunological diagnosis of schistosomiasis.
机译：我们使用可商购的抗原条带评估了蛋白质印迹（WB）分析的性能，并将结果与​​间接血凝（IHA）和间接免疫荧光（IFAT）的结果进行了人类血吸虫病的血清诊断。抗原制剂是曼氏血吸虫的粗提物。血吸虫病的WB分布特征通过比较来自寄生虫学证实为 S的58个血清样本的结果进行了表征。 mansoni （ n = 12）和 S。血吸虫病（ n = 46）感染和37例血吸虫病病例，但血清学检查结果均为阳性。通过检测健康受试者的12份血清样本，67份患有其他证明的蠕虫和原生动物感染的患者的血清样本以及16份自身抗体患者的血清样本来评估WB分析的特异性。从血吸虫病患者的血清中发现了六个免疫优势带（65、70、80、95、110和120 kDa）。除100-kDa条带外，三个或三个以上范围在65-120kDa的条带被认为可诊断血吸虫感染，在我们的系列中特异性为100％。在血吸虫病确诊患者中，WB分析的敏感性为84.5％，而IFAT和IHA的敏感性分别为65.5和72.9％。对于经血清学证实的病例，WB分析的敏感性为97.3％。两组患者的总体敏感性和特异性分别为89.5和100％，阳性和阴性预测值分别为100和91.3％。我们得出结论，WB分析是用于血吸虫病免疫学诊断的有用技术。

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