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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Comparison of Antibody Responses to a Potential Combination of Specific Glycolipids and Proteins for Test Sensitivity Improvement in Tuberculosis Serodiagnosis
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Comparison of Antibody Responses to a Potential Combination of Specific Glycolipids and Proteins for Test Sensitivity Improvement in Tuberculosis Serodiagnosis

机译：比较针对特定糖脂和蛋白质潜在结合的抗体反应，以改善结核病血清诊断的测试敏感性

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The humoral response to different proteinaceous antigens of Mycobacterium tuberculosis is heterogeneous among patients with active disease, and this has originated in the proposal to use a combination of several specific antigens to find an efficient serodiagnostic test for tuberculosis (TB). However, to date, comparisons of antibody responses to several antigens in the same population have been carried out without consideration of antigenic cell wall glycolipids. In the present study the presence of immunoglobulin G (IgG), IgM, and IgA antibodies to M. tuberculosis glycolipids (sulfolipid I, diacyltrehaloses, triacyltrehaloses, and cord factor) was compared with the response to four commercially available tests based on the 38-kDa protein mixed with the 16-kDa protein or lipoarabinomannan. Fifty-two serum samples from TB patients and 83 serum samples from control individuals (48 healthy individuals and 35 non-TB pneumonia patients) were studied. Three relevant results were obtained. (i) Smear-negative TB patients presented low humoral responses, but the sera which did react principally showed IgA antibodies to some glycolipidic antigens. (ii) TB patients exhibit heterogeneous humoral responses against glycolipidic antigens. (iii) Finally, test sensitivity is improved (from 23 to 62%) when IgG and IgA antibodies are detected together in tests based on different antigens (proteins and glycolipids). We conclude that it is possible to include glycolipidic antigens in a cocktail of specific antigens from M. tuberculosis to develop a serodiagnostic test.
机译：在患有活动性疾病的患者中，对结核分枝杆菌的不同蛋白质抗原的体液反应是异质的，这源于提议使用几种特定抗原的组合来找到一种有效的结核病血清诊断测试方法）。然而，迄今为止，已经在不考虑抗原性细胞壁糖脂的情况下进行了对相同群体中几种抗原的抗体应答的比较。在本研究中，存在针对 M的免疫球蛋白G（IgG），IgM和IgA抗体。将结核糖脂（硫脂I，二酰基海藻糖，三酰基海藻糖和脐带因子）与对基于38 kDa蛋白与16 kDa蛋白或lipoarabinomannan混合的四种市售测试的反应进行了比较。研究了来自结核病患者的52份血清样品和来自对照个体（48位健康个体和35位非结核性肺炎患者）的83份血清样品。获得了三个相关结果。 （i）涂片阴性结核病患者的体液反应低，但是主要反应的血清显示针对某些糖脂抗原的IgA抗体。 （ii）结核病患者对糖脂抗原表现出异质的体液反应。 （iii）最后，在基于不同抗原（蛋白质和糖脂）的测试中同时检测到IgG和IgA抗体时，测试灵敏度提高了（从23％到62％）。我们得出结论，有可能在来自 M的特定抗原混合物中包括糖脂抗原。肺结核以进行血清学诊断测试。

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