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首页> 外文期刊>Journal of Clinical Microbiology >Clinical Evaluation of Anti-Tuberculous Glycolipid Immunoglobulin G Antibody Assay for Rapid Serodiagnosis of Pulmonary Tuberculosis
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Clinical Evaluation of Anti-Tuberculous Glycolipid Immunoglobulin G Antibody Assay for Rapid Serodiagnosis of Pulmonary Tuberculosis

机译：抗结核性糖脂免疫球蛋白G抗体检测快速诊断肺结核的临床评价

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摘要

Previously we reported the development of a highly sensitive enzyme-linked immunosorbent assay specific for anti-tuberculous glycolipid (anti-TBGL) for the rapid serodiagnosis of tuberculosis. In this study, the usefulness of an anti-TBGL antibody assay kit for rapid serodiagnosis was evaluated in a controlled multicenter study. Antibody titers in sera from 318 patients with active pulmonary tuberculosis (216 positive for Mycobacterium tuberculosis in smear and/or culture tests and 102 smear and culture negative and clinically diagnosed), 58 patients with old tuberculosis, 177 patients with other respiratory diseases, 156 patients with nonrespiratory diseases, and 454 healthy subjects were examined. Sera from 256 younger healthy subjects from among the 454 healthy subjects were examined as a control. When the cutoff point of anti-TBGL antibody titer was determined as 2.0 U/ml, the sensitivity for active tuberculosis patients was 81.1% and the specificity was 95.7%. Sensitivity in patients with smear-negative and culture-negative active pulmonary tuberculosis was 73.5%. Even in patients with noncavitary minimally advanced lesions, the positivity rate (60.0%) and the antibody titer (4.6 ± 9.4 U/ml) were significantly higher than those in the healthy group. These results indicate that this assay using anti-TBGL antibody is useful for the rapid serodiagnosis of active pulmonary tuberculosis.
机译：先前，我们报道了针对肺结核快速血清诊断的抗结核糖脂（anti-TBGL）特异的高灵敏度酶联免疫吸附测定方法的开发。在这项研究中，在一项受控的多中心研究中评估了抗TBGL抗体检测试剂盒对快速血清诊断的有效性。 318例活动性肺结核患者的血清抗体滴度（涂片和/或培养试验中结核分枝杆菌阳性216例，临床诊断为涂片和培养阴性102例），老年肺结核58例，177例与其他呼吸系统疾病一起，检查了156例非呼吸系统疾病患者和454位健康受试者。检查了454名健康受试者中256名较年轻健康受试者的血清作为对照。当抗TBGL抗体滴度的临界点确定为2.0 U / ml时，活动性结核病患者的敏感性为81.1％，特异性为95.7％。涂片阴性和培养阴性的活动性肺结核患者的敏感性为73.5％。即使在无空洞的轻微晚期病变患者中，阳性率（60.0％）和抗体滴度（4.6±9.4 U / ml）也显着高于健康组。这些结果表明，使用抗TBGL抗体进行的这项测定可用于活动性肺结核的快速血清诊断。

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