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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Comparison of PCR-generated fragments of the mce gene from Mycobacterium tuberculosis, M. avium, M. intracellulare, and M. scrofulaceum.
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Comparison of PCR-generated fragments of the mce gene from Mycobacterium tuberculosis, M. avium, M. intracellulare, and M. scrofulaceum.

机译：PCR生成的结核分枝杆菌，鸟分枝杆菌，胞内分枝杆菌和s分枝杆菌mce基因片段的比较。

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摘要

Nineteen strains representing 13 species of mycobacteria were tested for the ability to serve as PCR templates for the production of a 293-bp fragment of the mycobacterial mce gene. The mce gene is a virulence factor recently sequenced from Mycobacterium tuberculosis. PCR products were obtained for only the species of the Mycobacterium tuberculosis complex (MTC) and the Mycobacterium avium-M. intracellulare-M. scrofulaceum complex. The fragment was sequenced from M. tuberculosis (one strain), M. avium (three strains), M. intracellulare (two strains), and M. scrofulaceum (two strains). Sequence comparisons suggest that the fragments from each of the species are regions that code for a similar product. One of the M. scrofulaceum strains yielded a sequence whose most probable reading frame was truncated by an amber stop codon caused by a single nuclei acid difference from the other sequences. The amino acid sequences from the non-MTC sequences cluster together, displaying fewer differences from each other than from the M. tuberculosis sequence and the truncated M. scrofulaceum sequence. Principal component analysis of the distance matrix displays the clustering of the M. avium and M. intracellulare sequences into single-species clusters. It is concluded that at least one open reading frame of the mce gene is found, although it is discernibly different, in pathogenic mycobacteria other than the MTC.
机译：测试了代表13种分枝杆菌的19个菌株的能力，以用作产生分枝杆菌mce基因的293bp片段的PCR模板。 mce基因是最近从结核分枝杆菌测序的毒力因子。仅针对结核分枝杆菌复合体（MTC）和鸟分枝杆菌-M的物种获得了PCR产物。细胞内阴囊复杂。该片段从结核分枝杆菌（一个菌株），鸟分枝杆菌（三个菌株），胞内分枝杆菌（两个菌株）和霉（两个菌株）测序。序列比较表明，每个物种的片段都是编码相似产物的区域。 s霉的一种菌株产生了一个序列，其最可能的阅读框被琥珀终止密码子截断，该琥珀终止密码子是由与其他序列的单个核酸差异引起的。来自非MTC序列的氨基酸序列聚在一起，彼此之间的差异小于与结核分枝杆菌序列和被截短的阴囊支原体序列的差异。距离矩阵的主成分分析显示鸟分枝杆菌和细胞内分枝杆菌序列聚类为单一物种簇。结论是，在除MTC之外的致病性分枝杆菌中发现了至少一个mce基因的开放阅读框，尽管可以明显地发现其不同。

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