Detection of serum immunoglobulin M to human cytomegalovirus by western blotting correlates better with virological data than detection by conventional enzyme immunoassay.

T Lazzarotto; G T Maine; P Del Monte; H Frush; K Shi; M P Landini

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Detection of serum immunoglobulin M to human cytomegalovirus by western blotting correlates better with virological data than detection by conventional enzyme immunoassay.

机译：通过蛋白质印迹法检测人巨细胞病毒的血清免疫球蛋白M与病毒学数据的相关性比通过常规酶免疫测定法更好。

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Western blotting (immunoblotting) with proteins separated from purified human cytomegalovirus (HCMV) particles (viral WB) has repeatedly been shown to be a reliable and sensitive method for detecting HCMV-specific immunoglobulin M (IgM). The aim of the present work was to determine whether IgM detected by viral WB correlates with virological diagnosis better than conventional enzyme immunoassay (conv-EIA). The presence of an active HCMV infection was documented on the basis of isolation of virus from urine and/or saliva and on the basis of antigenemia and/or PCR with polymorphonuclear leukocytes for immunocompetent and immunocompromised subjects, respectively. The agreement observed between IgM detected by viral WB and the results obtained by virological detection of HCMV was significantly higher (88.7%) than the agreement of IgM detected by conv-EIA and virological results (67.5%).

机译：从纯化的人巨细胞病毒（HCMV）颗粒（病毒WB）分离的蛋白质的蛋白质印迹（免疫印迹）已被反复证明是检测HCMV特异性免疫球蛋白M（IgM）的可靠且灵敏的方法。本工作的目的是确定病毒WB检测到的IgM与病毒学诊断是否比常规酶免疫测定（conv-EIA）更好。分别从尿液和/或唾液中分离病毒，以及针对多能核白细胞进行抗原血症和/或PCR分别针对免疫能力强和免疫功能低下的受试者，证明了存在活动性HCMV感染。病毒WB检测到的IgM与HCMV病毒学检测结果之间的一致性显着高于conv-EIA检测到的IgM与病毒学结果的一致性（67.5％）（88.7％）。

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《Clinical and diagnostic laboratory immunology》 |1996年第5期|共4页
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T Lazzarotto; G T Maine; P Del Monte; H Frush; K Shi; M P Landini;
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Detection of serum immunoglobulin M to human cytomegalovirus by western blotting correlates better with virological data than detection by conventional enzyme immunoassay.

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