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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Assessment of a New Immunoassay for Serological Confirmation and Discrimination of Human T-Cell Lymphotropic Virus Infections
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Assessment of a New Immunoassay for Serological Confirmation and Discrimination of Human T-Cell Lymphotropic Virus Infections

机译：血清学确认和歧视人类T细胞淋巴病毒感染的一种新的免疫测定的评估。

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The present study evaluated a new confirmatory assay for antibodies to human T-cell lymphotropic virus type 1 and 2 (HTLV-1 and HTLV-2) proteins performed with serum samples from various commercial sources. The new test is a line immunoassay (LIA) with a nylon membrane sensitized with the most relevant antigens of HTLVs: the envelope gp46 and gp21 as well as the gag p24 and p19 antigens, represented by either recombinant proteins or synthetic peptides. A total of 176 serum or plasma samples were tested, of which 66 were HTLV-1 positive, 72 were HTLV-2 positive, and 38 were HTLV negative; of the 38 HTLV-negative samples 23 were indeterminate by Western blotting (WB). Serially diluted samples (n = 33) from HTLV-1- and HTLV-2-infected patients were also analyzed to determine the sensitivity of the new assay. The new confirmatory assay (INNO-LIA HTLV) performed markedly better than WB assays for those samples reactive by screening. Accurate confirmation of the presence of HTLV-1 and HTLV-2 antibodies and accurate discrimination of HTLV-1 and HTLV-2 antibodies were obtained for all the HTLV-seropositive samples. Due to its enhanced specificity and sensitivity, the new assay not only improves the ability to confirm and discriminate HTLV infections but also eliminates the vast majority of WB-indeterminate and false-positive specimens.
机译：本研究评估了一种新的验证性测定法，该测定法是对来自各种商业来源的血清样本进行的1型和2型人T细胞淋巴病毒（HTLV-1和HTLV-2）蛋白抗体的检测。新的测试是一种线免疫测定（LIA），其尼龙膜对HTLV的最相关抗原敏感：包膜gp46和gp21以及 gag p24和p19抗原，由两种重组蛋白代表或合成肽。总共测试了176份血清或血浆样本，其中HTLV-1阳性66份，HTLV-2阳性72份，HTLV阴性38份; 38个HTLV阴性样品中的23个通过Western blotting（WB）不确定。还分析了来自HTLV-1和HTLV-2感染患者的系列稀释样品（ n = 33），以确定新测定的敏感性。对于那些通过筛选具有反应性的样品，新的确证分析（INNO-LIA HTLV）的性能明显优于WB分析。对于所有HTLV血清反应阳性的样本，均能准确确认HTLV-1和HTLV-2抗体的存在，并能准确区分HTLV-1和HTLV-2抗体。由于其增强的特异性和敏感性，新的测定方法不仅提高了确定和区分HTLV感染的能力，而且消除了绝大多数WB不明确和假阳性的标本。

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