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Human T-cell lymphotropic virus type II envelope protein and human monoclonal antibodies specific therefor

机译:人类T细胞淋巴病毒II型包膜蛋白及其特异性单克隆抗体

摘要

Isolated and purified envelope protein of HTLV-I is provided devoid of non-envelope protein of HTLV-I and having substantially the same conformation as the envelope protein in native HTLV-I. The protein is produced recombinantly using a dual vaccinia/T7 polymerase system. Non-glycosylated and glycosylated forms of the protein are produced. Glycosylated forms are recognized by antibodies specific for the envelope protein of HTLV-I. Monoclonal antibodies are provided which are specific for the HTLV-I envelope protein and non-binding to HTLV-I envelope protein in denatured form. The HTLV-I envelope protein is cross-reactive with antibodies of HTLV-II and STLV. The envelope protein is useful in diagnosis of infection by HTLV-I and HTLV-II.
机译:提供了分离和纯化的HTLV-1的包膜蛋白,其不含HTLV-1的非包膜蛋白,并且具有与天然HTLV-1中的包膜蛋白基本相同的构象。使用双重牛痘/ T7聚合酶系统重组生产该蛋白。产生蛋白质的非糖基化和糖基化形式。糖基化形式被特异于HTLV-1的包膜蛋白的抗体识别。提供了对HTLV-1包膜蛋白具有特异性并且不以变性形式与HTLV-1包膜蛋白结合的单克隆抗体。 HTLV-I包膜蛋白与HTLV-II和STLV的抗体发生交叉反应。包膜蛋白可用于诊断HTLV-I和HTLV-II感染。

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