Comparison of Polymorphonuclear Cells from Healthy Donors and Differentiated HL-60 Cells as Phagocytes in an Opsonophagocytic Assay Using Antigen-Coated Fluorescent Beads

Bruno Guy; Catherine Testart; Sophie Gimenez; Violette Sanchez; Philippe Lheritier; Danielle Rossin

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Comparison of Polymorphonuclear Cells from Healthy Donors and Differentiated HL-60 Cells as Phagocytes in an Opsonophagocytic Assay Using Antigen-Coated Fluorescent Beads

机译：使用抗原包被的荧光微珠在调理吞噬试验中比较健康供体的多形核细胞和分化为吞噬细胞的HL-60细胞

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Polymorphonuclear cells (PMNs) from healthy donors and differentiated HL-60 cells were compared in an opsonophagocytic assay using fluorescent latex beads coated with Streptococcus pneumoniae polysaccharide conjugates. Serum-specific phagocytosis was efficiently mediated by both sources of cells, as measured by flow cytometry, but the mean number of beads ingested per cell was three- to fivefold higher when PMNs were used than when HL-60 cells were used. Nevertheless, differentiated HL-60 cells could be a convenient and standardized source of cells to evaluate the functionality of specific antibodies to vaccine candidates as a coating on fluorescent beads.

机译：使用涂有肺炎链球菌多糖缀合物的荧光乳胶珠在调理吞噬试验中比较健康供体的多形核细胞（PMN）和分化的HL-60细胞。通过流式细胞术测量，两种细胞来源均能有效地介导血清特异性吞噬作用，但使用PMN时，每个细胞摄入的平均珠粒数比使用HL-60细胞时高三到五倍。然而，分化的HL-60细胞可能是方便，标准化的细胞来源，用于评估针对候选疫苗的特异性抗体作为荧光珠涂层的功能。

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《Clinical and diagnostic laboratory immunology》 |2000年第2期|共4页
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Bruno Guy; Catherine Testart; Sophie Gimenez; Violette Sanchez; Philippe Lheritier; Danielle Rossin;
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专利

1. Standardization of an opsonophagocytic assay for the measurement of functional antibody activity against Streptococcus pneumoniae using differentiated HL-60 cells. [J] . S Romero-Steiner, D Libutti, L B Pais, Clinical and diagnostic laboratory immunology . 1997,第4期

机译：使用分化的HL-60细胞测量抗肺炎链球菌功能抗体活性的调理吞噬测定法的标准化。
2. Development of an Opsonophagocytic Killing Assay Using HL-60 Cells for Detection of Functional Antibodies against Streptococcus pyogenes [J] . Sanaz Salehi, Claudia M. Hohn, Thomas A. Penfound, mSphere . 2018,第6期

机译：使用HL-60细胞检测化脓性链球菌功能抗体的调理吞噬杀伤试验的开发。
3. Phenotypic and Functional Analysis of HL-60 Cells Used in Opsonophagocytic-Killing Assay for Streptococcus pneumoniae [J] . Kim Kyung-Hyo, Seoh Ju Young, Cho Su Jin Journal of Korean medical science . 2015,第2期

机译：肺炎链球菌调理吞噬法检测HL-60细胞的表型和功能分析
4. Flow cytometric, phase-resolved discrimination of damaged/dead cells by propidium iodide uptake in macrohages having phagocytized fluorescent microspheres [C] . John A. Steinkamp, Yolanda E. Valdez, Bruce E. Lehnert Conference on Advances in Fluorescence Sensing Technology Ⅴ Jan 24-25, 2001, San Jose, USA . 2001

机译：在具有吞噬作用的荧光微球的巨噬细胞中通过碘化丙啶摄取对受损/死细胞进行流式细胞术，相分辨判别
5. A study of human homologs of selected Drosophila genes in human mammary ductal carcinoma, asymmetric cell division of human neural stem cells and a multiplex microsphere bead assay for comparative RNA expression analysis. [D] . Fuja, Tannin J. 2003

机译：对人类乳腺导管癌中所选果蝇基因的人类同源物，人类神经干细胞的不对称细胞分裂以及用于比较RNA表达分析的多重微球珠分析的研究。
6. Note: Comparison of Polymorphonuclear Cells from Healthy Donors and Differentiated HL-60 Cells as Phagocytes in an Opsonophagocytic Assay Using Antigen-Coated Fluorescent Beads [O] . Bruno Guy, Catherine Testart, Sophie Gimenez, 2000

机译：注意：使用抗原包被的荧光珠在调理吞噬试验中比较健康供体的多形核细胞和作为吞噬细胞的分化HL-60细胞
7. Comparison of Polymorphonuclear Cells from Healthy Donors and Differentiated HL-60 Cells as Phagocytes in an Opsonophagocytic Assay Using Antigen-Coated Fluorescent Beads [O] . Guy, Bruno, Testart, Catherine, Gimenez, Sophie, 2000

机译：使用抗原包被的荧光微珠在调理吞噬试验中比较健康供体的多形核细胞和分化为吞噬细胞的HL-60细胞

Comparison of Polymorphonuclear Cells from Healthy Donors and Differentiated HL-60 Cells as Phagocytes in an Opsonophagocytic Assay Using Antigen-Coated Fluorescent Beads

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