An IS711 Element Downstream of thebp26 Gene Is a Specific Marker of Brucella spp. Isolated from Marine Mammals

Axel Cloeckaert; Maggy Grayon; Olivier Grepinet

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An IS711 Element Downstream of thebp26 Gene Is a Specific Marker of Brucella spp. Isolated from Marine Mammals

机译：bp26基因下游的IS711元件是布鲁氏菌属的特异性标记。与海洋哺乳动物隔离

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DNA polymorphism of the bp26 gene, coding for a diagnostic protein antigen for brucellosis, was assessed by PCR and restriction fragment length polymorphism analysis using primers to amplify the bp26 gene with its flanking regions. Surprisingly, whereas PCR performed on DNA of the reference strains of the six recognized Brucella species produced a product of the expected size (1,029 bp), PCR performed on DNA of three representative strains from marine mammals (from a seal, a dolphin, and a porpoise) produced a larger product, of about 1,900 bp. Nucleotide sequencing of the 1,900-bp PCR products revealed the presence of an insertion sequence, IS711, downstream of thebp26 gene and adjacent to a Bru-RS1 element previously described as being a hot spot for IS711 insertion. PCR performed on a large number of field strains from different geographic origins and from marine mammal isolates indicated that the occurrence of an IS711 element downstream of the bp26 gene was a feature specific to the marine mammal Brucellastrains. Thus, this PCR assay is able to differentiateBrucella terrestrial isolates from marine mammal isolates and could be applied for diagnostic purposes.

机译：通过PCR和限制性片段长度多态性分析，使用引物扩增 bp26 基因及其侧翼，评估编码布鲁氏菌病诊断蛋白抗原的 bp26 基因的DNA多态性。地区。出乎意料的是，虽然对六个公认的 Brucella 物种的参考菌株的DNA进行PCR产生了预期大小（1,029 bp）的产物，但是对三种来自海洋哺乳动物的代表性菌株的DNA进行了PCR（来自海豹，海豚和海豚）产生了大约1900 bp的较大产物。 1,900 bp PCR产物的核苷酸测序表明，在 bp26 基因下游且与先前描述为Bru-RS1元件相邻的插入序列IS 711 是IS 711 插入的热点。对来自不同地理起源和海洋哺乳动物分离株的大量野外菌株进行的PCR表明，在 bp26 基因下游的IS 711 元件的出现是特定于特征的到海洋哺乳动物 Brucella 菌株。因此，该PCR测定法能够从海洋哺乳动物分离物中区分出陆生小球藻，并可以用于诊断目的。 展开▼

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《Clinical and diagnostic laboratory immunology》 |2000年第5期|共5页

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Axel Cloeckaert; Maggy Grayon; Olivier Grepinet;
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中图分类诊断学;

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专利

1. Novel IS711 Chromosomal Location Useful for Identification of Marine Mammal Brucella Genotype ST27, Which Is Associated with Zoonotic Infection [J] . Axel Cloeckaert, Nelly Bernardet, Mark S. Koylass, Journal of Clinical Microbiology . 2011,第11期

机译：新颖的IS711染色体定位可用于鉴定与人畜共患病感染有关的海洋哺乳动物布鲁氏菌基因型ST27

2. Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus. [J] . Cloeckaert A, Verger JM, Grayon M, Microbes and infection . 2001,第9期

机译：布鲁氏菌属的分类。通过在omp2基因座处的DNA多态性从海洋哺乳动物中分离得到。

3. Identification of an IS711 Element Interrupting the wboA Gene of Brucella abortusVaccine Strain RB51 and a PCR Assay To Distinguish Strain RB51 from Other Brucella Species and Strains [J] . Ramesh Vemulapalli, John R. McQuiston, Gerhardt G. Schurig, Clinical and diagnostic laboratory immunology . 1999,第5期

机译：中断流产布鲁氏菌疫苗RB51的wboA基因的IS711元件的鉴定和区别其他布鲁氏菌属和菌株的RB51的PCR分析

4. Brucella infections in marine mammals from scottish waters [C] . I. A. P. Patterson, F. E. Howie, R. J. Reid Annual Conference of the American Association of Zoo Veterinarians . 2000

机译：来自苏格兰水域的海洋哺乳动物的Brucella感染

5. The use of selective microbial insecticides for control of specific field generations of Heliothis spp. on cotton and economic injury levels and damage levels for specific field generations of Heliothis spp. on cotton [D] . Halford, William Tadmore 1987

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6. Note: An IS711 Element Downstream of the bp26 Gene Is a Specific Marker of Brucella spp. Isolated from Marine Mammals [O] . Axel Cloeckaert, Maggy Grayon, Olivier Grepinet 2000

机译：注意：bp26基因下游的IS711元素是布鲁氏菌属的特异性标记。与海洋哺乳动物隔离

7. An IS711 Element Downstream of the bp26 Gene Is a Specific Marker of Brucella spp. Isolated from Marine Mammals [O] . Cloeckaert, Axel, Grayon, Maggy, Grepinet, Olivier 2000

机译：bp26基因下游的IS711元件是布鲁氏菌属的特异性标记。与海洋哺乳动物隔离

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4. 呼和浩特布鲁氏菌分离株bp26基因的原核表达 [J] . 李建云 ,张忠兵 ,王宇 . 内蒙古大学艺术学院学报 . 2012,第004期

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6. 布鲁氏菌外膜蛋白bp26基因的克隆、表达及活性鉴定 [C] . . 首届中国兽药大会暨中国畜牧兽医学会动物药品学分会2008年学术年会 . 2008

7. 布鲁氏菌标记疫苗(M5--90--26)的鉴别诊断及其bp26蛋白的免疫原性分析 [A] . 刘文兴 . 2011

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3. isolated nucleic acid molecule, vector, genetically modified cell, isolated helicoveipa spp. chymotrypsin, antagonist, composition, genetically modified plant, seed or other reproductive material, methods for modulating hpch5 activity or a homologue or variant thereof in an insect , to modulate expression of hpf5 or homolog or variant in an insect, to isolate and separate individual chymotrypsin isoforms, to select a chymotrypsin antagonist insensitive to helicoverpa spp., and, isolated inhibitor [P] . 外国专利： BRPI0409638A . 2006-04-25

机译：分离的核酸分子，载体，转基因细胞，分离的Helicoveipa spp。胰凝乳蛋白酶，拮抗剂，组合物，转基因植物，种子或其他生殖材料，调节昆虫中hpch5活性或其同源物或变体的方法，调节昆虫中hpf5或同源物或变体的表达，分离和分离单个胰凝乳蛋白酶的方法异构体，以选择对Helicoverpa spp。不敏感的胰凝乳蛋白酶拮抗剂，以及分离的抑制剂

4. SET OF PRIMERS, PROBES, PROCEDURE AND KIT FOR DETECTION AND QUANTIFICATION OF DNA SEQUENCES SPECIFIC TO BRUCELLA SPP. [P] . 外国专利： ES2331679A1 . 2010-01-12

机译：用于检测和定量布鲁氏菌属SPP特异DNA序列的试剂盒，探针，方法和试剂盒。

5. Set of primers, probes, method and kit for the detection and quantification of specific DNA sequences of Brucella spp. [P] . 外国专利： ES2331679B1 . 2010-09-30

机译：用于检测和定量布鲁氏菌属特定DNA序列的引物，探针，方法和试剂盒的组合。

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An IS711 Element Downstream of thebp26 Gene Is a Specific Marker of Brucella spp. Isolated from Marine Mammals

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