Clustering of Clinical Strains ofHelicobacter pylori Analyzed by Two-Dimensional Gel Electrophoresis

Helena Enroth; Thomas ?kerlund; Anna Sillén; Lars Engstrand

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Clustering of Clinical Strains ofHelicobacter pylori Analyzed by Two-Dimensional Gel Electrophoresis

机译：二维凝胶电泳分析幽门螺杆菌临床菌群

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Strain variations of Helicobacter pylori have been tested by numerous methods and compared among different patient groups. The aim of this study was to investigate whether H. pyloriexpresses disease-specific proteins that can be detected by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). H. pylori strains isolated from duodenal ulcer, gastric cancer, and gastritis patients were analyzed. Extensive variation in spot patterns was observed between the strains, but a dendrogram analysis revealed that some strains within each disease group clustered together. Eight proteins were sequenced and found in the H. pylori genome sequence. 2-D PAGE is a useful method for studies of protein expression and for highlighting the extensive strain variation that H. pylori exhibits.

机译：幽门螺杆菌的菌株变异已通过多种方法进行了测试，并在不同患者组之间进行了比较。这项研究的目的是调查是否 H。幽门螺杆菌表达疾病特异性蛋白，可通过二维聚丙烯酰胺凝胶电泳（2-D PAGE）检测到。 H。分析了从十二指肠溃疡，胃癌和胃炎患者中分离出的幽门螺杆菌菌株。菌株之间观察到斑点模式的广泛变化，但树状图分析显示每个疾病组中的某些菌株聚集在一起。对八个蛋白质进行了测序，并在 H中发现了它们。幽门螺杆菌基因组序列2-D PAGE是研究蛋白质表达并突出 H的广泛菌株变异的有用方法。幽门螺旋杆菌展览。 展开▼

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《Clinical and diagnostic laboratory immunology》 |2000年第2期|共6页

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Helena Enroth; Thomas ?kerlund; Anna Sillén; Lars Engstrand;
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中图分类诊断学;

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机译：通过二维凝胶电泳，序列分析和血清分析鉴定幽门螺杆菌的潜在诊断和疫苗候选者

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机译：二维凝胶电泳幽门螺杆菌临床菌株的对比蛋白质组分析

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机译：使用一维和二维凝胶电泳确定正常和两性男性小口黑鲈的差异蛋白表达谱。

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机译：注意：二维凝胶电泳分析幽门螺杆菌临床菌株的聚类。

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机译：二维凝胶电泳分析幽门螺杆菌临床菌株的聚类

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Clustering of Clinical Strains ofHelicobacter pylori Analyzed by Two-Dimensional Gel Electrophoresis

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